Mitophagy is primarily due to alternative autophagy and requires the MAPK1 and MAPK14 signaling pathways
| Autor: | Yusuke Kurihara, Tomotake Kanki, Xiulian Jin, Shun-ichi Yamashita, Tetsu Saigusa, Dongchon Kang, Masamune Aihara, Yuko Hirota |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Mitogen-Activated Protein Kinase 1
Carbonyl Cyanide m-Chlorophenyl Hydrazone Gene knockdown p38 mitogen-activated protein kinases Autophagy ATG5 Mitophagy Basic Science Research Papers Cell Biology Mitochondrion Biology Mitochondria Cell biology Mitogen-Activated Protein Kinase 14 ATG12 Mice Animals Humans Lysosomes Molecular Biology MAP1LC3B HeLa Cells Signal Transduction |
| Zdroj: | Autophagy. 11:332-343 |
| ISSN: | 1554-8635 1554-8627 |
| DOI: | 10.1080/15548627.2015.1023047 |
| Popis: | In cultured cells, not many mitochondria are degraded by mitophagy induced by physiological cellular stress. We observed mitophagy in HeLa cells using a method that relies on the pH-sensitive fluorescent protein Keima. With this approach, we found that mitophagy was barely induced by carbonyl cyanide m-chlorophenyl hydrazone treatment, which is widely used as an inducer of PARK2/Parkin-related mitophagy, whereas a small but modest amount of mitochondria were degraded by mitophagy under conditions of starvation or hypoxia. Mitophagy induced by starvation or hypoxia was marginally suppressed by knockdown of ATG7 and ATG12, or MAP1LC3B, which are essential for conventional macroautophagy. In addition, mitophagy was efficiently induced in Atg5 knockout mouse embryonic fibroblasts. However, knockdown of RAB9A and RAB9B, which are essential for alternative autophagy, but not conventional macroautophagy, severely suppressed mitophagy. Finally, we found that the MAPKs MAPK1/ERK2 and MAPK14/p38 were required for mitophagy. Based on these findings, we conclude that mitophagy in mammalian cells predominantly occurs through an alternative autophagy pathway, requiring the MAPK1 and MAPK14 signaling pathways. |
| Databáze: | OpenAIRE |
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