In vitro-activated tumor-bearing host T cells and the effectiveness of tumor vaccine immunotherapy
| Autor: | Ying Wang, Jun Ma, Yue-ling Wang, Cai-xia Feng, Yun-ping Wang, Shang-feng Gao, Qi-ling Li |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
genetic structures
animal diseases medicine.medical_treatment T-Lymphocytes lcsh:Medicine chemical and pharmacologic phenomena Cancer Vaccines Immunotherapy Adoptive Immune system Lymphocytes Tumor-Infiltrating Lymphopenia Medicine Animals Ovarian Neoplasms Antitumor immunity business.industry lcsh:R General Medicine Immunotherapy biochemical phenomena metabolism and nutrition In vitro Rats Vaccination Immunology bacteria Cytokines Female business |
| Zdroj: | Annals of Saudi Medicine, Vol 32, Iss 2, Pp 162-168 (2012) |
| ISSN: | 0975-4466 0256-4947 |
| DOI: | 10.5144/0256-4947.2012.162 |
| Popis: | BACKGROUND AND OBJECTIVE: Vaccination during periods of lymphopenia may facilitate immune responses to weak self-antigens and enhance antitumor immunity. The objective of this study was to determine the effectiveness of tumor vaccine immunotherapy combined with immune reconstruction using tumor-bearing host immune cells in lymphopenia, and to investigate the role of tumor-bearing host T cells activated in vitro during immunotherapy. DESIGN AND SETTING: Animal study conducted in the First Affiliated Hospital of Xi'an Jiaotong University from January 2009 to January 2010. PATIENTS AND METHODS: Lymphopenia was induced by cyclophosphamide. A reconstituted immune system with different syngeneic lymphocytes was employed, including lymphocytes from naÏve rats (unsensitized group), tumor-bearing rats (tumor-bearing group), and tumor-bearing rats activated in vitro (activated group). All rats were immunized with granulocyte-macrophage colony-stimulating factor (GM-CSF)-modified NuTu-19 ovarian cancer (GM-CSF/NuTu-19) cells. Tumor vaccine-draining lymph nodes (TVDLNs) were harvested, and then stimulated to induce effector T cells (TE). TE were then adoptively transferred to rats bearing a 3-day pre-established abdominal tumor (NuTu-19), and the survival rate was calculated. RESULTS: Compared with the unsensitized group, the levels of interleukin-2 (IL-2) were significantly lower in the tumor-bearing group, whereas that of IL-4 were significantly higher (P< .05). The number of CD4+ T cells secreting interferon-γ and the specific cytotoxicity of CD8+ cytotoxic T lymphocytes were significantly lower (P< .05). The survival was significantly higher in the activated group compared with the other groups. CONCLUSIONS: Lymphocytes from tumor-bearing rats activated in vitro can effectively reverse the immunosuppressive effects of tumor-bearing hosts. |
| Databáze: | OpenAIRE |
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