Leishmania chagasi: Homogenous metacyclic promastigotes isolated by buoyant density are highly virulent in a mouse model
| Autor: | Chaoqun Yao, Bayan Sudan, John E. Donelson, Mary E. Wilson, Yani Chen |
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| Rok vydání: | 2008 |
| Předmět: |
Immunology
Virulence Biology Glycosphingolipids Article Microbiology Mice chemistry.chemical_compound Cricetinae parasitic diseases Animals Parasite hosting Leishmania infantum Mice Inbred BALB C Kinetoplastida General Medicine Lipophosphoglycan Leishmania chagasi biology.organism_classification Leishmania In vitro Isoenzymes Disease Models Animal Infectious Diseases Liver chemistry Leishmaniasis Visceral Female Parasitology Spleen Peptide Hydrolases |
| Zdroj: | Experimental Parasitology. 118:129-133 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/j.exppara.2007.06.012 |
| Popis: | Homogenous metacyclic promastigotes of Leishmania chagasi were isolated by buoyant density from in vitro heterogeneous cultures and used for biochemical characterization of isoforms of the major surface protease (MSP). Compared to stationary phase promastigotes, metacyclic cells had three times more MSP, produced three-fold higher parasite loads in a mouse model in vivo, and were more resistant to complement-mediated lysis in vitro. These metacyclic L. chagasi expressed both the virulence-associated 59-kDa, and the constitutively expressed 63-kDa, isoforms of MSP. |
| Databáze: | OpenAIRE |
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