Existence of Multiple ESBL Genes among Phenotypically Confirmed ESBL Producing Klebsiella pneumoniae and Escherichia coli Concurrently Isolated from Clinical, Colonization and Contamination Samples from Neonatal Units at Bugando Medical Center, Mwanza, Tanzania
| Autor: | Stephen E. Mshana, Abubakar S. Hoza, Dory Kovacs, Katarina Oravcova, Vitus Silago, Louise Matthews, Jeremiah Seni, Happyness Samson, Athumani M. Lupindu |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Klebsiella pneumoniae medicine.drug_class 030106 microbiology Antibiotics extended-spectrum beta-lactamase RM1-950 medicine.disease_cause Biochemistry Microbiology 03 medical and health sciences Antibiotic resistance Multiplex polymerase chain reaction medicine polycyclic compounds Pharmacology (medical) Colonization antimicrobial resistance General Pharmacology Toxicology and Pharmaceutics Escherichia coli biology biochemical phenomena metabolism and nutrition biology.organism_classification bacterial infections and mycoses 030104 developmental biology Infectious Diseases bacteria Therapeutics. Pharmacology Mobile genetic elements Bacteria |
| Zdroj: | Antibiotics, Vol 10, Iss 476, p 476 (2021) Antibiotics Volume 10 Issue 5 |
| ISSN: | 2079-6382 |
| Popis: | The proportions and similarities of extended-spectrum β-lactamase (ESBL) producing K. pneumoniae (ESBL-KP) and E. coli (ESBL-EC) carrying multiple ESBL genes is poorly known at our setting. This study investigated the existence of multiple ESBL genes (blaCTX-M, blaTEM, and blaSHV) among ESBL-KP and ESBL-EC concurrently isolated from clinical, colonization, and contamination samples from neonatology units in Mwanza-Tanzania. Twenty and 55 presumptive ESBL-EC and ESBL-KP, respectively, from a previous study archived at −80 °C were successfully recovered for this study. Isolates were screened and confirmed for production of ESBLs by phenotypic methods followed by multiplex PCR assay to determine ESBL genes. All (100%) and 97.3% of presumptive ESBL isolates were phenotypically confirmed by Clinical and Laboratory Standards Institute (CLSI) and modified double-disc synergy methods, respectively. About 93.3% (70/75) of phenotypically confirmed ESBL isolates had at least one ESBL gene, whereby for 62.9% (44/70), all ESBL genes (blaCTX-M, blaTEM, and blaSHV) were detected. Eight pairs of ESBL bacteria show similar patterns of antibiotics susceptibility and ESBL genes. ESBL-KP and ESBL-EC, concurrently isolated from clinical, colonization and contamination samples, harbored multiple ESBL genes. Further, eight pairs of ESBL isolates had similar patterns of antibiotics susceptibility and ESBL genes, suggesting transmission of and/or sharing of mobile genetic elements (MGEs) among ESBL-KP and ESBL-EC. |
| Databáze: | OpenAIRE |
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