The Potential Application of Spring Sargassum glaucescens Extracts in the Moisture-Retention of Keratinocytes and Dermal Fibroblast Regeneration after UVA-Irradiation
| Autor: | Liu Fu Chen, Ciao-Ting Chen, Yi-Tsen Lin, Kan Kai-Wen, Li Zih-Yi, Hsin-Fen Hsu, Yu-Ting Lin, Yung-Hsiang Lin, Hsiang-Ling Su, Chin-Hsiu Yu |
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| Rok vydání: | 2019 |
| Předmět: |
Aging
antioxidant Antioxidant medicine.medical_treatment Cell anti-photoaging Pharmaceutical Science wound healing Dermatology lcsh:Chemistry Superoxide dismutase Dermal fibroblast 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Keratin medicine Chemical Engineering (miscellaneous) moisture-retention 030304 developmental biology chemistry.chemical_classification Sargassum glaucescens 0303 health sciences Reactive oxygen species CCD-966SK fibroblasts integumentary system biology Chemistry Cell growth human primary epidermal keratinocytes Molecular biology medicine.anatomical_structure lcsh:QD1-999 biology.protein Surgery Wound healing |
| Zdroj: | Cosmetics, Vol 6, Iss 1, p 17 (2019) |
| ISSN: | 2079-9284 |
| Popis: | Sargassum glaucescens is a marine brown alga with high antioxidant activity. To evaluate the potential application of Sargassum glaucescens extracts (SGE) in skincare, we performed in vitro assays in dermal fibroblasts and epidermal keratinocytes. The antioxidant activity of SGE was confirmed by the suppression of H2O2-induced reactive oxygen species (ROS) production in dermal fibroblasts and in vitro 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity. In the wound healing assay, application of 2 mg/ml SGE stimulated the wound closure of CCD-966SK fibroblasts by a 2.95-fold in comparison to the control. Furthermore, treatment with SGE of concentrations ranging from 0.25 to 1 mg/ml promoted CCD-966SK cell regeneration after UVA irradiation. At the molecular level, 1 mg/ml SGE induced expressions of anti-oxidative genes SOD1 (Superoxide dismutase 1) and GPX1 (Glutathione peroxidase 1), and DNA repair regulatory genes XRCC1 (X-ray repair cross-complementing protein 1) and ERCC6 (Excision repair cross-complementation Group 6) in CCD-966SK cells after UVA irradiation. Therefore, SGE displayed beneficial effects on cell regeneration and the protection of dermal cells against UVA irradiation. In epidermal cells, SGE stimulated the cell proliferation of human primary epidermal keratinocytes. Application of 0.03125 mg/ml SGE induced the expressions of skin barrier-related genes TGM1 (Transglutaminase 1), KRT10 (Keratin 10) and KRT14 in keratinocytes. Meanwhile, SGE induced the gene expression of FLG (Filaggrin), which promoted the production of natural moisturizing factor (NMF) for maintaining the moisture and barrier functions of skin. |
| Databáze: | OpenAIRE |
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