Microbiome pattern of Lucilia sericata (Meigen) (Diptera: Calliphoridae) and feeding substrate in the presence of the foodborne pathogen Salmonella enterica
| Autor: | Iulia Roxana Angelescu, Cristina Purcarea, Carlos Henríquez-Castillo, Victoria Ioana Paun, Paris Lavin, Lavinia Iancu |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Firmicutes Science 030106 microbiology Human pathogen medicine.disease_cause Lucilia Article Microbiology 03 medical and health sciences medicine Animals Microbiome Pathogen Multidisciplinary biology Diptera Microbiota fungi Salmonella enterica Pathogenic bacteria Feeding Behavior biology.organism_classification 030104 developmental biology Medicine Metagenomics Proteobacteria |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-021-94761-w |
| Popis: | The microbial diversity and quantitative dynamics during the insect’s development stages constitute recently developed putative tools in forensic and medical studies. Meanwhile, little is known on the role of insects in spreading foodborne pathogenic bacteria and on the impact of these pathogens on the overall insects and feeding substrate microbiome composition. Here, we provide the first characterization of the bacterial communities harbored in adult and immature stages of Lucilia sericata, one of the first colonizers of decomposed human remains, in the presence of the foodborne pathogen Salmonella enterica using 16S rRNA Illumina sequencing and qPCR. The pathogen transmission from the wild adults to the second generation was observed, with a 101.25× quantitative increase. The microbial patterns from both insect and liver samples were not influenced by the artificial introduction of this pathogenic foodborne bacteria, being dominated by Firmicutes and Proteobacteria. Overall, our results provided a first detailed overview of the insect and decomposed substrate microbiome in the presence of a human pathogen, advancing the knowledge on the role of microbes as postmortem interval estimators and the transmission of pathogenic bacteria. |
| Databáze: | OpenAIRE |
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