Comparative Analysis of Multiplex Platforms for Detecting Vitreous Biomarkers in Diabetic Retinopathy
| Autor: | Suzette Farber-Katz, Tong Zhao, Gulesi Ayanoglu, Jay M. Stewart, Paul E. Carrington, Catherine Psaras, Franklin Vives, Sawarin Laotaweerungsawat, Santosh C. Sutradhar, Yi Chen, Nina Xiaoyan Li, Audrey Phone, Ricardo Lamy, Dahui Ma |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Proteomics Biomedical Engineering Biology Article vitreous 03 medical and health sciences 0302 clinical medicine Opthalmology and Optometry medicine Diabetes Mellitus cytokine Humans Multiplex Eye Disease and Disorders of Vision Rank correlation Diabetic Retinopathy Diabetes Diabetic retinopathy medicine.disease Molecular biology eye diseases 4.1 Discovery and preclinical testing of markers and technologies Vitreous Body Ophthalmology 030104 developmental biology cytokine biomarker 030221 ophthalmology & optometry Cytokines biomarker sense organs Detection rate Protein abundance Protein concentration Biomarkers |
| Zdroj: | Translational vision science & technology, vol 9, iss 10 Translational Vision Science & Technology |
| Popis: | Author(s): Lamy, Ricardo; Farber-Katz, Suzette; Vives, Franklin; Ayanoglu, Gulesi; Zhao, Tong; Chen, Yi; Laotaweerungsawat, Sawarin; Ma, Dahui; Phone, Audrey; Psaras, Catherine; Li, Nina Xiaoyan; Sutradhar, Santosh; Carrington, Paul E; Stewart, Jay M | Abstract: PurposeTo evaluate the feasibility of using the Proximity Extension Assay (PEA) platform to detect biomarkers in vitreous and to compare the findings with results obtained with an electrochemiluminescent (ECL) sandwich immunoassay.MethodsVitreous samples from patients with proliferative diabetic retinopathy (PDR) and non-diabetic controls were tested using two different proteomics platforms. Forty-one assays were completed with the ECL platform and 459 with the PEA platform. Spearman's rank correlation coefficient (rs ) was used to determine the direction and strength of the relationship between protein levels detected by both platforms.ResultsThree hundred sixty-six PEA assays detected the tested protein in at least 25% of samples, and the difference in protein abundance between PDR and controls was statistically significant for 262 assays. Seventeen ECL assays yielded a detection rate ≥ 25%, and the difference in protein concentration between PDR and controls was statistically significant for 13 proteins. There was a subset of proteins that were detected by both platforms, and for those the Spearman's correlation coefficient was higher than 0.8.ConclusionsPEA is suitable for the analysis of vitreous samples, showing a strong correlation with the ECL platform. The detection rate of PEA panels was higher than the panels tested with ECL. The levels of several proinflammatory and angiogenic cytokines were significantly higher in PDR vitreous compared to controls.Translational relevanceThis study provides new information on the yields of small-volume assays that can detect proteins of interest in ocular specimens, and it identifies patterns of cytokine dysregulation in PDR. |
| Databáze: | OpenAIRE |
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