2′-(R)-Fluorinated mC, hmC, fC and caC triphosphates are substrates for DNA polymerases and TET-enzymes
| Autor: | Franziska R. Traube, Edris Parsa, Arne S. Schröder, Thomas Carell, Sascha Serdjukow, Katharina Iwan, Miriam Wallner |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Halogenation DNA polymerase Context (language use) Cytidine DNA-Directed DNA Polymerase 010402 general chemistry 01 natural sciences Catalysis Dioxygenases Epigenesis Genetic Substrate Specificity Nucleobase 03 medical and health sciences Polyphosphates Materials Chemistry Humans Epigenetics Demethylation chemistry.chemical_classification biology Chemistry HEK 293 cells Metals and Alloys DNA General Chemistry Base excision repair 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials HEK293 Cells 030104 developmental biology Enzyme Biochemistry Ceramics and Composites biology.protein |
| Zdroj: | Chemical Communications. 52:14361-14364 |
| ISSN: | 1364-548X 1359-7345 |
| Popis: | A deeper investigation of the chemistry that occurs on the newly discovered epigenetic DNA bases 5-hydroxymethyl-(hmdC), 5-formyl-(fdC), and 5-carboxy-deoxycytidine (cadC) requires chemical tool compounds, which are able to dissect the different potential reaction pathways in cells. Here we report that the 2'-(R)-fluorinated derivatives F-hmdC, F-fdC, and F-cadC, which are resistant to removal by base excision repair, are good substrates for DNA polymerases and TET enzymes. This result shows that the fluorinated compounds are ideal tool substances to investigate potential C-C-bond cleaving reactions in the context of active demethylation. |
| Databáze: | OpenAIRE |
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