Understanding the role of chromatin remodeling in the regulation of circadian transcription in Drosophila
| Autor: | Rosanna S. Kwok, Vu H. Lam, Joanna C. Chiu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Transcription Genetic Circadian clock CLOCK Proteins RNA polymerase II Cell Cycle Proteins histone Chromatin remodeling Cell Line Promoter Regions 03 medical and health sciences 0302 clinical medicine Genetic circadian clock Transcriptional regulation Genetics Nucleosome Animals Drosophila Proteins Promoter Regions Genetic Transcription factor Brahma Evolutionary Biology biology Ecology Extra View Chromatin Assembly and Disassembly SWI/SNF Chromatin Cell biology Circadian Rhythm 030104 developmental biology Drosophila melanogaster Gene Expression Regulation Insect Science biology.protein Trans-Activators gene expression transcription 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Fly Fly, vol 9, iss 4 |
| ISSN: | 1933-6942 |
| Popis: | Circadian clocks enable organisms to anticipate daily changes in the environment and coordinate temporal rhythms in physiology and behavior with the 24-h day-night cycle. The robust cycling of circadian gene expression is critical for proper timekeeping, and is regulated by transcription factor binding, RNA polymerase II (RNAPII) recruitment and elongation, and post-transcriptional mechanisms. Recently, it has become clear that dynamic alterations in chromatin landscape at the level of histone posttranslational modification and nucleosome density facilitate rhythms in transcription factor recruitment and RNAPII activity, and are essential for progression through activating and repressive phases of circadian transcription. Here, we discuss the characterization of the BRAHMA (BRM) chromatin-remodeling protein in Drosophila in the context of circadian clock regulation. By dissecting its catalytic vs. non-catalytic activities, we propose a model in which the non-catalytic activity of BRM functions to recruit repressive factors to limit the transcriptional output of CLOCK (CLK) during the active phase of circadian transcription, while the primary function of the ATP-dependent catalytic activity is to tune and prevent over-recruitment of negative regulators by increasing nucleosome density. Finally, we divulge ongoing efforts and investigative directions toward a deeper mechanistic understanding of transcriptional regulation of circadian gene expression at the chromatin level. |
| Databáze: | OpenAIRE |
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