The ATPase Irc20 facilitates Rad51 chromatin enrichment during homologous recombination in yeast Saccharomyces cerevisiae
| Autor: | Deena Jalal, Mehwish Iqbal, Jisha Chalissery, Ahmed H. Hassan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Saccharomyces cerevisiae Proteins
DNA damage DNA repair RAD52 Saccharomyces cerevisiae RAD51 Biochemistry 03 medical and health sciences 0302 clinical medicine Adenosine Triphosphate DNA Breaks Double-Stranded DNA Fungal Molecular Biology 030304 developmental biology 0303 health sciences biology DNA Helicases Recombinational DNA Repair Cell Biology biology.organism_classification Ubiquitin ligase Chromatin Cell biology Rad52 DNA Repair and Recombination Protein 030220 oncology & carcinogenesis biology.protein Rad51 Recombinase Homologous recombination |
| Zdroj: | DNA repair. 97 |
| ISSN: | 1568-7856 |
| Popis: | DNA double-strand breaks (DSBs) constitute one of the most cytotoxic forms of DNA damage and pose a significant threat to cell viability, survival, and homeostasis. DSBs have the potential to promote aneuploidy, cell death and potentially deleterious mutations that promote tumorigenesis. Homologous recombination (HR) is one of the main DSB repair pathways and while being essential for cell survival under genotoxic stress, it requires proper regulation to avoid chromosome rearrangements. Here, we characterize the Saccharomyces cerevisiae E3 ubiquitin ligase/putative helicase Irc20 as a regulator of HR. Using purified Irc20, we show that it can hydrolyze ATP in the presence and absence of DNA, but does not increase access to DNA within a nucleosome. In addition, we show that both the ATPase and ubiquitin ligase activities of Irc20 are required for suppressing the spontaneous formation of recombination foci. Finally, we demonstrate a role for Irc20 in promoting Rad51 chromatin association and the removal of Rad52 recombinase from chromatin, thus facilitating subsequent HR steps and directing recombination to more error-free modes. |
| Databáze: | OpenAIRE |
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