Subtype identification of human Blastocystis spp. isolated from Lao People's Democratic Republic
| Autor: | Issarapong Phosuk, Tongjit Thanchomnang, Sakhone Laymanivong, Wanchai Maleewong, Pewpan M. Intapan, Rutchanee Rodpai, Lakkhana Sadaow, Oranuch Sanpool |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Adolescent Veterinary (miscellaneous) 030231 tropical medicine LAO PEOPLE'S DEMOCRATIC REPUBLIC Blastocystis Infections DNA Ribosomal Polymerase Chain Reaction law.invention Microbiology 03 medical and health sciences Feces Young Adult 0302 clinical medicine Phylogenetics law Prevalence Animals Humans Child Polymerase chain reaction Phylogeny Aged Aged 80 and over Blastocystis Traditional medicine biology Phylogenetic tree 030108 mycology & parasitology Ribosomal RNA Middle Aged biology.organism_classification Infectious Diseases Molecular Diagnostic Techniques Laos Insect Science Molecular phylogenetics Parasitology Female |
| Zdroj: | Acta tropica. 168 |
| ISSN: | 1873-6254 |
| Popis: | Blastocystis sp. is the most common protist found in the intestinal tract of humans and animals. Blastocystis subtypes (STs) are classified based on the molecular phylogeny of the small subunit rRNA gene (SSU rDNA). At least 17 Blastocystis STs have been reported and, of these, STs 1-9 have been found in humans. This study revealed the presence of human Blastocystis STs in Lao People's Democratic Republic (Lao PDR). Fecal samples were collected from apparently healthy people from the central province of Khammouane and the southern province of Champasak. Fresh fecal samples, found to be positive for Blastocystis using microscopy, were individually cultured in Jones' medium and each sample was used for the amplification and sequencing of a fragment of SSU rDNA. BLAST searches and phylogenetic analysis confirmed the distribution of four Blastocystis STs: ST1 (64%), ST2 (8%), ST3 (24%) and ST7 (4%). This is the first report to provide molecular data revealing the prevalence of Blastocystis STs in apparently healthy people from Lao PDR. |
| Databáze: | OpenAIRE |
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