An assessment of candidate genes to assist prognosis in gastric cancer
| Autor: | Kishore Gopalakrishnan, Hisham Mehenna, Chuka U. Nwokolo, Ramesh P. Arasaradnam, Adrian Gelsthorpe, Michael McFarlane, Janusz Jankowski, Julia Brettschneider, Sean James, David Snead |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Oncology Candidate gene medicine.medical_specialty business.industry Mortality rate Gastroenterology Cancer Retrospective cohort study A300 medicine.disease 03 medical and health sciences 030104 developmental biology Internal medicine Gene expression medicine Biomarker (medicine) Immunohistochemistry Original Article business Gene |
| ISSN: | 2078-6891 |
| Popis: | Gastric cancer (GC) is the fourth commonest cancer worldwide, with the second highest mortality rate. Its poor mortality is linked to delayed presentation. There is a drive towards non-invasive biomarker screening and monitoring of many different types of cancer, although with limited success so far. We aimed to determine if any genes from a 32-gene panel could be used to determine GC prognosis. We carried out a retrospective study on the expression of 32 genes, selected for their proven or potential links to GC, on historic formalin fixed paraffin-embedded (FFPE) GC specimens from our unit. Gene expression was measured using quantitative nuclease protection assays (qNPA) technology. Following statistical analysis of the results, immunohistochemical staining for eight genes, both discriminating and non-discriminating, was conducted in seven age and sex matched non-metastatic: metastatic GC pairings. The stained samples were reviewed by two blinded consultant histopathologists. Multivariate Cox analysis of the gene expression data revealed metastatic status, age, sex and five genes appeared to influence GC survival. Genes negatively influencing survival included and (relative risks 2.20, 3.73 and 7.53 respectively). Genes conveying survival benefit included and (relative risks 0.10 and 0.24 respectively). Immunohistochemical staining of seven age and sex matched non-metastatic: metastatic pairs revealed no association between gene expression and protein expression. Our study found several genes whose expression may affect GC prognosis. However, immunohistochemical analysis revealed no association between gene expression and protein expression. It remains to be determined whether gene expression or protein expression are reliable means of assessing GC prognosis. |
| Databáze: | OpenAIRE |
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