Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks
| Autor: | Michelle Durkin, Sherry F. Queener, N. L. Bauer, J. W. Smith, Chao-Hung Lee, Marilyn S. Bartlett, Margaret M. Shaw |
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| Jazyk: | angličtina |
| Rok vydání: | 1993 |
| Předmět: |
Microbiology (medical)
Molecular Sequence Data Mycology Biology Polymerase Chain Reaction Microbiology Cell Line Rats Sprague-Dawley Laboratory flask chemistry.chemical_compound Antigen Animals Humans DNA Fungal Lung Base Sequence Pneumocystis Microcarrier Fibroblasts biology.organism_classification Embryo Mammalian Embryonic stem cell Rats chemistry Pneumocystis carinii Cell culture Evaluation Studies as Topic Protozoa Female DNA Research Article |
| Popis: | A method of growing rat Pneumocystis carinii with human embryonic lung fibroblasts (HEL-299 cells) sheeted onto microcarrier beads has been developed. This method allows production of large quantities of P. carinii organisms with very little contamination of host cells. A fivefold increase in the numbers of organisms was achieved, as determined by organism count, antigen detection, and DNA quantification. The majority of organisms produced by this method are trophozoites. |
| Databáze: | OpenAIRE |
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