Relationship of Matrix Metalloproteinases and Macrophages to Embolization During Endoluminal Carotid Interventions
| Autor: | Robert D. Goldin, Colin Bicknell, Nawar A. Alkhamesi, Nicholas J.W. Cheshire, John H.N. Wolfe, Rodney A. Foale, Ara Darzi, M.W. Clark, Michael Jenkins, David H. Peck, Mark G. Cowling |
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| Rok vydání: | 2004 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.medical_treatment Embolism Gelatin Zymography Carotid endarterectomy In Vitro Techniques Matrix metalloproteinase Angioplasty medicine Humans Gelatinase Carotid Stenosis Radiology Nuclear Medicine and imaging Embolization CD68 business.industry Macrophages Models Cardiovascular Tissue Inhibitor of Metalloproteinases Matrix Metalloproteinases Pulsatile Flow Surgery Cardiology and Cardiovascular Medicine business Carotid Artery Internal Ex vivo |
| Zdroj: | Journal of Endovascular Therapy. 11:483-493 |
| ISSN: | 1545-1550 1526-6028 |
| Popis: | PURPOSE To investigate if relationships exist among macrophage infiltration, plasma matrix metalloproteinase (MMP) levels, and the number of emboli generated during endoluminal carotid interventions. METHODS Carotid endarterectomy specimens excised as intact cylinders (n=27) were subjected to a standardized angioplasty procedure under radiological guidance in an ex vivo pulsatile flow model. Emboli collected in distal filters were counted and sized using microscopy. Preoperative plasma gelatinase activity was determined by gelatin zymography and quantified using image analysis software. Levels of tissue inhibitors of metalloproteinases (TIMP) 1 and 2 were determined by ELISA. Macrophages within postangioplasty plaques were analyzed using immunohistochemical staining for CD68 antigen and graded by a blinded examiner. Statistical analysis was performed using Spearman's rank correlation. RESULTS The median number of emboli recorded during angioplasty was 104 (interquartile range 33.75-242.5, absolute range 13-1090). Plasma MMP-9 and MMP-2 levels correlated with emboli number (r=0.544 [p=0.003] and r=0.412, [p=0.033], respectively), while TIMP-1 and TIMP-2 levels did not. Macrophage infiltration within the plaques correlated with emboli number (r=0.722, p |
| Databáze: | OpenAIRE |
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