Free calcium in full grown Xenopus laevis oocyte following treatment with ionophore A 23187 or progesterone
| Autor: | René Ozon, J. Stinnakre, Robert Bellé |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
medicine.medical_specialty
Xenopus Ionophore Aequorin Stimulation Biochemistry Membrane Potentials Oogenesis Endocrinology Internal medicine medicine Animals Molecular Biology Calcimycin Progesterone Ovum Membrane potential biology biology.organism_classification Oocyte Anti-Bacterial Agents Membrane medicine.anatomical_structure Free calcium Oocytes Biophysics biology.protein Calcium Female Chloromercuribenzoates |
| Zdroj: | Molecular and Cellular Endocrinology. 8:65-72 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/0303-7207(77)90018-1 |
| Popis: | Free intracellular Ca 2+ was monitored in isolated Xenopus laevis oocyte during induced maturation using the Ca 2+ -sensitive luminescent protein, aequorin. Internal free Ca 2+ was not precisely measured but data suggest it was quite low (in the micromolar range). No change in internal free Ca 2+ was detected during maturation induced either by progesterone or by p -chloromercuribenzoate. By contrast, the ionophore A 23187 gave an increase in the free Ca 2+ level when there was a raised external Ca 2+ (10 mM), conditions which also induce oocyte maturation. About 3 h after progesterone or p -chloromercuribenzoate stimulation, the oocyte membrane potential decreased by about 50 mV while the membrane resistance increased transitorily. |
| Databáze: | OpenAIRE |
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