Analysis of Correlation between the Seven Important Helicobacter pylori (H. pylori) Virulence Factors and Drug Resistance in Patients with Gastritis
| Autor: | Nogayhan Seymen, Yeşim Gürol, Elif Kilic, Ugur Sezerman, Zehra Cekic-Kipritci, Nesteren Mansur-Ozen, Sinem Oktem-Okullu |
|---|---|
| Přispěvatelé: | Acibadem University Dspace |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Article Subject Virulence Drug resistance RC799-869 Microbiology 03 medical and health sciences 0302 clinical medicine Antibiotic resistance Clarithromycin Genotype medicine CagA Hepatology biology business.industry Gastroenterology Helicobacter pylori Diseases of the digestive system. Gastroenterology biology.organism_classification bacterial infections and mycoses 030104 developmental biology 030211 gastroenterology & hepatology Gastritis medicine.symptom business medicine.drug |
| Zdroj: | Gastroenterology Research and Practice, Vol 2020 (2020) |
| ISSN: | 1687-6121 |
| Popis: | The aim of this study is to evaluate the association between seven important H. pylori virulence factors and antibiotic resistance in patients with gastritis. H. pylori strains isolated from 33 patients with gastritis were examined. Antimicrobial susceptibilities were tested by GenoType® HelicoDR (Hain Life Science, Germany) test kit and RT-PCR. The virulence-factors were determined using conventional PCR. 39% of patients were resistant for clarithromycin and 27% of patients were resistant for fluoroquinolone. 15% of patients were resistant to both clarithromycin and fluoroquinolone. The H. pylori vacA m1/s2 genotype was the most frequent allelic combination. Patients were possessed the vacA s1, m1 (6.1%); s1, m2 (6.1%); s2, m1 (15.1%); and s2, m2 (3.0%) genotypes. 94% of patients with gastritis were positive for H. pylori napA gene. Also, there were no dupA gene-positive gastritis patients. There was no significant correlation between the vacA, cagA, oipA, hpaA, babA, napA, dupA, ureA, ureB virulence genes, clarithromycin, and fluoroquinolone resistance. Herein, we report that the relationship between the H. pylori napA gene and gastritis. Although we found a correlation between H. pylori virulence factor and clinical outcome, there is a need for further studies to enlighten the relation between H. pylori virulence genes and antibiotic resistance. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text