Separation of Sympathomimetic Amines of Abuse and Related Compounds by Micellar Electrokinetic Chromatography
| Autor: | Daisuke Sato, Naotaka Kuroda, Kenichiro Nakashima, Yuji Nakahara, Kaname Ohyama, Mitsuhiro Wada |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Detection limit
Reproducibility Chromatography Tea Illicit Drugs Chemistry Sodium Relative standard deviation Sodium Dodecyl Sulfate chemistry.chemical_element General Chemistry General Medicine sympathomimetic amine phentermine Micellar electrokinetic chromatography Sympathomimetic Amines Calibration Drug Discovery micellar electrokinetic chromatography Chinese tea Sympathomimetics Sodium dodecylsulfate Chromatography Micellar Electrokinetic Capillary |
| Zdroj: | Chemical & Pharmaceutical Bulletin. 49(7):905-908 |
| ISSN: | 0009-2363 |
| Popis: | Separation of twelve sympathomimetic amines and related compounds by micellar electrokinetic chromatography (MEKC) with UV absorbance detection is described. These amines were well separated within 25 min using 50 mM sodium tetraborate solution containing 15 mM sodium dodecylsulfate (SDS) of pH 9.3 as a running solution and detected at 210 nm. MEKC was performed with an applied voltage of 13 kV at 25 °C using a fused-silica capillary (50 cm×75 mm i.d.) with effective length of 37.5 cm. The detection limits of these compounds were in the range from 4 to 97 fmol/injection at a signal-to-noise ratio (S/N) of 3. The reproducibility of the method expressed as relative standard deviation (RSD) for within-day (n=6) and between-day (n=5) assays was less than 4.8 and 8.8%, respectively. The proposed method could be applied to the determination of an anorectic drug, phentermine, in Chinese tea with a detection limit of 99 μg/g (105 fmol/injection, S/N=3). Chemical & Pharmaceutical Bulletin v.49(7) p.905-908, 2001 |
| Databáze: | OpenAIRE |
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