Inhibition of ovarian cancer cell proliferation by Pien Tze Huang via the AKT-mTOR pathway
| Autor: | Xin Zhang, Mu Yan Cai, Shuang‑Bo Tang, Quan Wan, Chang‑Peng Li, Hui‑Ni Wu, Jian‑Ding Cheng, Fan He |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
biology endocrine system diseases business.industry Cell growth proliferation Cell Cancer Articles Cell cycle medicine.disease medicine.anatomical_structure ovarian cancer Oncology Cyclin-dependent kinase Immunology medicine biology.protein Cancer research cell cycle Cyclin-dependent kinase 6 business Protein kinase B PI3K/AKT/mTOR pathway Pien Tze Huang |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1082 1792-1074 |
| Popis: | Pien Tze Huang (PZH) is a well-known Chinese medicine that has been used as a therapeutic drug in the treatment of a number of diseases, such as hepatocellular carcinoma and colon cancer. However, few studies have analyzed the effects of PZH on ovarian cancer cell proliferation. In the present study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Transwell assays, cell cycle and apoptosis rate analyses and western blotting were conducted to investigate the effects of PZH on the proliferation rate of ovarian cancer cells and its potential molecular pathway. The results showed that PZH inhibits the proliferation of the human ovarian cancer OVCAR-3 cell line by blocking the progression of the cell cycle from the G1 to S phase, however, PZH did not induce OVCAR-3 cell apoptosis. Increased PZH concentration may downregulate the expression of AKT, phosphorylated (p)-AKT, mammalian target of rapamycin (mTOR) and p-mTOR proteins in the OVCAR-3 cell line. In addition, it was observed that PZH may suppress the protein expression of cyclin-dependent kinase (CDK)4 and CDK6. Overall, the results of the present study indicated that PZH may inhibit ovarian cancer cell proliferation by modulating the activity of the AKT-mTOR pathway. |
| Databáze: | OpenAIRE |
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