Local and Systemic Immune Responses to Influenza A Virus Infection in Pneumonia and Encephalitis Mouse Models
| Autor: | Masato Yashiro, Yoshiharu Nagaoka, Hirokazu Tsukahara, Yosuke Washio, Nobuyuki Nosaka, Kenji Baba, Tsuneo Morishima, Mutsuko Yamada |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Chemokine Article Subject medicine.medical_treatment Pneumonia Viral Clinical Biochemistry medicine.disease_cause Virus Mice 03 medical and health sciences Influenza A Virus H1N1 Subtype 0302 clinical medicine Immune system Orthomyxoviridae Infections Genetics medicine Influenza A virus Animals Encephalitis Viral Molecular Biology lcsh:R5-920 medicine.diagnostic_test biology business.industry Biochemistry (medical) General Medicine medicine.disease Virology respiratory tract diseases Mice Inbred C57BL Pneumonia 030104 developmental biology Bronchoalveolar lavage Cytokine Immunology biology.protein Cytokines lcsh:Medicine (General) business Bronchoalveolar Lavage Fluid 030217 neurology & neurosurgery Encephalitis Research Article |
| Zdroj: | Disease Markers, Vol 2017 (2017) Disease Markers |
| ISSN: | 1875-8630 0278-0240 |
| DOI: | 10.1155/2017/2594231 |
| Popis: | Objective. To compare local and systemic profiles between different disease pathologies (pneumonia and encephalitis) induced by influenza A virus (IAV).Methods. An IAV pneumonia model was created by intranasal inoculation of C57BL/6 mice with influenza A/WSN/33 (H1N1) virus. Lung lavage and blood collection were performed on day 3 after IAV inoculation. Similarly, an IAV encephalitis mouse model was created by direct intracranial IAV inoculation. Cerebrospinal fluid (CSF) and blood collection were conducted according to the same schedule. Cytokine/chemokine profiles were produced for each collected sample. Then the data were compared visually using radar charts.Results. Serum cytokine profiles were similar in pneumonia and encephalitis models, but local responses between the bronchoalveolar lavage fluid (BALF) in the pneumonia model and CSF in the encephalitis model differed. Moreover, to varying degrees, the profiles of local cytokines/chemokines differed from those of serum in both the pneumonia and encephalitis models.Conclusion. Investigating local samples such as BALF and CSF is important for evaluating local immune responses, providing insight into pathology at the primary loci of infection. Serum data alone might be insufficient to elucidate local immune responses and might not enable clinicians to devise the most appropriate treatment strategies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|