Rapid assay for lysyl-protocollagen hydroxylase activity
| Autor: | Ronald L. Miller |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Male
Lysyl-protocollagen hydroxylase Proline Tritiated water Guinea Pigs Lysine Biophysics Carrageenan Tritium Biochemistry Mixed Function Oxygenases Guinea pig chemistry.chemical_compound Rapid assay Methods Animals Chemical Precipitation Molecular Biology Skin Granuloma Chromatography Chemistry Protocollagen Cell Biology Chromatography Ion Exchange Rats Kinetics Hydroxylysine Ammonium Sulfate Isotope Labeling Collagen |
| Zdroj: | Analytical Biochemistry. 45:202-210 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(72)90020-6 |
| Popis: | A method for the assay of lysyl-protocollagen hydroxylase activity is described. This method depends upon the formation of tritiated water when lysine residues of 4,5-3H-lysyl-protocollagen are hydroxylated. The labeled protocollagen was prepared from carrageenan induced guinea pig granuloma tissue. There was a linear relationship between the amount of tritiated water and tritiated hydroxylysine formed in this assay. The assay is reproducible and more rapid than previously described assays for lysyl-protocollagen hydroxylase. Use of this method will facilitate further studies on collagen hydroxylysine formation. |
| Databáze: | OpenAIRE |
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