Development and application of a LC-HRMS/MS method for analyzing antihypertensive drugs in oral fluid for monitoring drug adherence
| Autor: | Felix Mahfoud, Michael Böhm, Ingrid Kindermann, Markus R. Meyer, Cathy M. Jacobs, Lilian H.J. Richter |
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| Rok vydání: | 2019 |
| Předmět: |
Drug
Analyte media_common.quotation_subject Ion suppression in liquid chromatography–mass spectrometry 02 engineering and technology Urine 01 natural sciences Biochemistry Analytical Chemistry Medication Adherence Matrix (chemical analysis) Tandem Mass Spectrometry Environmental Chemistry Humans Spectroscopy Antihypertensive Agents media_common Detection limit Chromatography Chemistry 010401 analytical chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences Body Fluids Adherence monitoring Oral fluid Drug Monitoring 0210 nano-technology Chromatography Liquid |
| Zdroj: | Analytica chimica acta. 1070 |
| ISSN: | 1873-4324 |
| Popis: | Nonadherence to antihypertensive drugs therapy is known to be a serious issue in hypertension treatment. Liquid chromatography (LC) coupled to mass spectrometry (MS) was shown to allow the assessment of such nonadherence in blood and urine sample. However, their sampling may represent a logistical challenge and are often not favored by the patients. We questioned whether oral fluid (OF) might be an easier accessible alternative matrix for adherence monitoring of cardiovascular drugs (CD). A qualitative method for adherence monitoring of 78 commonly prescribed cardiovascular drugs in OF using LC high-resolution MS (LC-HRMS/MS) was therefore developed, validated, and used to study the presence of antihypertensive medication in OF. Selectivity, ion suppression and enhancement due coeluting analytes, carry over, limits of detection (LOD), limits of identification (LOI), recovery (RE), matrix effects (ME), and process efficiency (PE) were investigated. For demonstrating applicability, over 50 OF samples were investigated and data were compared to findings in blood and urine. Selectivity in OF was given for all compounds via their MS2 spectra and no total suppression of signals could be observed. Determined LOI in OF for ten analytes was higher than the given therapeutic plasma concentration. Furthermore, RE, ME, and PE were in acceptable ranges for more than 65% of the compounds. In total, 208 prescriptions of CD to 57 patients were analyzed and demonstrated the suitability of for adherence monitoring in principle. OF was comparable to plasma regarding the drug categories and the frequencies of hits, except for acidic compounds but more hits could be found in urine samples. A analytical method using OF as analytical matrix was successfully developed. Application showed that it might be a suitable alternative for adherence monitoring of selected drugs in the future, particularly those having no acidic function. |
| Databáze: | OpenAIRE |
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