Exosomal microRNA signatures in multiple sclerosis reflect disease status
| Autor: | Saeideh Ebrahimkhani, Suzy S. J. Hur, Catherine M. Suter, Heidi N. Beadnall, Michael E. Buckland, Simon Hawke, Emma Devenney, Paul E. Young, Michael Barnett, Fatemeh Vafaee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Central Nervous System Male Pathology medicine.medical_specialty Small RNA Cell type lcsh:Medicine Biology Exosomes DNA sequencing Article 03 medical and health sciences Multiple Sclerosis Relapsing-Remitting microRNA medicine Humans lcsh:Science Multidisciplinary Base Sequence Sequence Analysis RNA Multiple sclerosis lcsh:R RNA High-Throughput Nucleotide Sequencing Middle Aged Multiple Sclerosis Chronic Progressive medicine.disease Microvesicles MicroRNAs 030104 developmental biology Gene Expression Regulation Immunology lcsh:Q Female Progressive disease |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) |
| ISSN: | 2045-2322 |
| Popis: | Multiple Sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system (CNS). There is currently no single definitive test for MS. Circulating exosomes represent promising candidate biomarkers for a host of human diseases. Exosomes contain RNA, DNA, and proteins, can cross the blood-brain barrier, and are secreted from almost all cell types including cells of the CNS. We hypothesized that serum exosomal miRNAs could present a useful blood-based assay for MS disease detection and monitoring. Exosome-associated microRNAs in serum samples from MS patients (n = 25) and matched healthy controls (n = 11) were profiled using small RNA next generation sequencing. We identified differentially expressed exosomal miRNAs in both relapsing-remitting MS (RRMS) (miR-15b-5p, miR-451a, miR-30b-5p, miR-342-3p) and progressive MS patient sera (miR-127-3p, miR-370-3p, miR-409-3p, miR-432-5p) in relation to controls. Critically, we identified a group of nine miRNAs (miR-15b-5p, miR-23a-3p, miR-223-3p, miR-374a-5p, miR-30b-5p, miR-433-3p, miR-485-3p, miR-342-3p, miR-432-5p) that distinguished relapsing-remitting from progressive disease. Eight out of nine miRNAs were validated in an independent group (n = 11) of progressive MS cases. This is the first demonstration that microRNAs associated with circulating exosomes are informative biomarkers not only for the diagnosis of MS, but in predicting disease subtype with a high degree of accuracy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|