C-phycocyanin suppresses transforming growth factor-β1-induced epithelial mesenchymal transition in human epithelial cells
| Autor: | Rajasekaran Subbiah, Rajaguru Palanichamy, Dhamotharan Pattarayan, Sivanantham Ayyanar, Dheeran Rajarajan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Epithelial-Mesenchymal Transition Cell cycle checkpoint Breast Neoplasms Vimentin Adenocarcinoma Transforming Growth Factor beta1 03 medical and health sciences medicine Humans Epithelial–mesenchymal transition Cell Proliferation Pharmacology A549 cell biology Cell growth Phycocyanin Epithelial Cells Cell Cycle Checkpoints General Medicine Epithelium Cell biology Fibronectin 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation A549 Cells MCF-7 Cells biology.protein Female Transforming growth factor |
| Zdroj: | Pharmacological Reports. 69:426-431 |
| ISSN: | 1734-1140 |
| Popis: | Epithelial mesenchymal transition (EMT) is a process through which epithelial cells undergo multiple biochemical changes, causing them to differentiate into a mesenchymal-cell phenotype. This process has been shown to contribute to the development of fibrotic diseases. C-phycocyanin (C-PC) is a phycobiliprotein extracted from Spirulina platensis. This study was done to investigate the effect of C-PC on transforming growth factor-β1 (TGF-β1)-induced EMT and an EMT associated proliferation in human epithelial cell lines. Human adenocarcinoma cell line, A549 and breast cancer cell line, MCF-7 were treated with TGF-β1, and EMT-related genes expression, cell proliferation and cell cycle arrest were examined. C-PC suppressed the EMT as assessed by reduced expression of vimentin, type-1-collagen and fibronectin, and increased E-cadherin expression in TGF-β1 treated cells. Further, TGF-β1 treatment induced cell cycle arrest in S and G2/M phase in A549 cells. However, TGF-β1-mediated cell cycle arrest was significantly reversed by combined treatment with C-PC. The overall data suggested that C-PC suppresses TGF- β1-induced EMT and warrants further in vivo studies for future evaluation of C-PC as a potential antifibrotic agent. |
| Databáze: | OpenAIRE |
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