Localization of IL-1?? mRNA and Cell Adhesion Molecules in the Maxillary Sinus Mucosa of Patients With Chronic Sinusitis
| Autor: | Miharu Ushikai, Eiichiro Tokushige, Kazunori Itoh, Katsunori Fukuda, Shoko Katahira |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Maxillary sinus Neutrophils medicine.medical_treatment E-selectin medicine Humans RNA Messenger Sinusitis Cells Cultured In Situ Hybridization Mucous Membrane biology business.industry Cell adhesion molecule Chronic sinusitis Intercellular Adhesion Molecule-1 Maxillary Sinusitis medicine.disease Immunohistochemistry medicine.anatomical_structure Cytokine Otorhinolaryngology Chronic Disease biology.protein E-Selectin business Cell Adhesion Molecules Infiltration (medical) Interleukin-1 |
| Zdroj: | The Laryngoscope. 104:1245 |
| ISSN: | 0023-852X |
| DOI: | 10.1288/00005537-199410000-00011 |
| Popis: | Interleukin-1 beta (IL-1 beta) is a predominant cytokine in retained paranasal sinus fluid of chronic sinusitis where infiltration by polymorphonuclear neutrophils (PMNs) of nasal and paranasal mucosa is characteristic. The authors investigated the localization of IL-1 beta messenger RNA (mRNA) in the maxillary sinus mucosa of patients with chronic sinusitis, using digoxigenin-labeled oligonucleotide probes. IL-1 beta mRNA was detected in some extravascular PMNs and small numbers of mononuclear leukocytes but was not detected in other tissue cells or intravascular leukocytes. The expression and distribution of the cell adhesion molecules, intercellular adhesion molecule-1 (ICAM-1) and endothelial-leukocyte adhesion molecule-1 (ELAM-1), were also studied in cultured human mucosal microvascular endothelial cells and in the maxillary sinus mucosa in chronic sinusitis by immunohistochemistry using monoclonal antibodies against these cell adhesion molecules. Only ICAM-1 was expressed on cultured human mucosal microvascular endothelial cells without IL-1 beta stimulation. With IL-1 beta activation of these cells, ELAM-1 was expressed strongly and the expression of ICAM-1 was enhanced. In the maxillary sinus mucosa, ICAM-1 was strongly and universally expressed on endothelial cells of all small vessels, whereas ELAM-1 was expressed only in the subepithelial region. These findings suggest that IL-1 beta, one of mediators in chronic sinusitis, is produced by PMNs, induces the expression of ICAM-1 and ELAM-1 on endothelial cells, and, thereby, stimulates PMN infiltration in chronic sinusitis. |
| Databáze: | OpenAIRE |
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