Cross Validation of HS-GC/MS to Quantify Total Carbon Dioxide in Horse Plasma
| Autor: | Jennifer Bash, A. Karl Larsen, Brendan Heffron |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
040301 veterinary sciences
Calibration curve Health Toxicology and Mutagenesis Instrumentation Analytical chemistry Toxicology 01 natural sciences Gas Chromatography-Mass Spectrometry Cross-validation Analytical Chemistry 0403 veterinary science chemistry.chemical_compound Calibration Animals Environmental Chemistry Horses Doping in Sports Chemical Health and Safety Chromatography Plasma samples Chemistry 010401 analytical chemistry Reproducibility of Results Horse 04 agricultural and veterinary sciences Carbon Dioxide Reference Standards 0104 chemical sciences Carbon dioxide Gas chromatography–mass spectrometry |
| Zdroj: | Journal of Analytical Toxicology. |
| ISSN: | 1945-2403 0146-4760 |
| DOI: | 10.1093/jat/bkw133 |
| Popis: | The use of alkinizing agents by trainers to counteract the accumulation of lactic acid in racehorses has been well documented. A by-product of this administration is elevated total carbon dioxide (tCO2) concentrations. Most regulatory authorities have set the threshold for tCO2 in plasma at 37 mM. The quantification of tCO2 often presents a challenge to laboratories due to the instrumentation required, which can be expensive to use and maintain. The Beckman DxC 600 (Brea, CA) is currently used in our laboratory for tCO2 quantification. The goal of this research was to determine if another analytical method could be used for this procedure. We report the use of headspace gas chromatography-mass spectrometry (HS-GC/MS) as an acceptable alternative to the use of the Beckman DxC 600. A six-point calibration curve ranging from 10 to 60 mM was analyzed along with controls at 15, 25 and 45 mM. Imprecision was found to be |
| Databáze: | OpenAIRE |
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