Pyridoxine analysis by high performance liquid chromatography and validation in fortified milk powder

Autor:	C.O. Amaro, C. Flores, Fernando C. Lidon, M.G. Dias
Rok vydání:	2014
Předmět:	Detection limit Accuracy and precision Analyte Pyridoxal Chromatography Calibration curve Pyridoxine Composição de Alimentos High-performance liquid chromatography chemistry.chemical_compound Method Validation Vitamin B6 chemistry medicine Vitamer HPLC Pyridoxamine Food Science medicine.drug
Zdroj:	Acta Alimentaria. 43:297-305
ISSN:	1588-2535 0139-3006
DOI:	10.1556/aalim.43.2014.2.14
Popis:	Vitamin B6 analysis by reverse-phase high performance liquid chromatography and its validation in milk powder prompted this study. The calibration curves for pyridoxal, pyridoxamine and pyridoxine were evaluated and method precision and accuracy assessed. It was found that the working range was adequate for all the analytes. The analytical procedure was verified by testing the enzymatic efficiency, precision and accuracy. In milk powder samples pyridoxine was the only vitamer found in quantities above the considered limit of quantification and the accuracy showed Z-scores lower than 2. Uncertainty estimation based on method validation results was 0.066 mg/100g, for a vitamin B6 content of 0.61 mg/100 g. It was concluded that the method is adequate for vitamin B6 quantification in powder milk.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef7c3132b3442d41c0f5f102dbbdbe79 https://doi.org/10.1556/aalim.43.2014.2.14 Zobrazit plný text záznamu