Perfusion promotes endothelialized pore formation in high concentration fibrin gels otherwise unsuitable for tube development
| Autor: | Axel Haverich, Sarah Zippusch, Florian Helms, Ulrike Böer, Melanie Klingenberg, Mathias Wilhelmi, Claudia Schrimpf, Skadi Lau |
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| Přispěvatelé: | University of Zurich, Böer, Ulrike |
| Rok vydání: | 2020 |
| Předmět: |
0206 medical engineering
Biomedical Engineering Medicine (miscellaneous) 2204 Biomedical Engineering Bioengineering 610 Medicine & health 02 engineering and technology Fibrin Biomaterials 03 medical and health sciences Re-Epithelialization Absorbable Implants Humans Tube (fluid conveyance) Volume concentration 030304 developmental biology High concentration 0303 health sciences Tissue engineered biology 1502 Bioengineering Tissue Engineering Tissue Scaffolds Chemistry Guided Tissue Regeneration 2502 Biomaterials 2701 Medicine (miscellaneous) Hydrogels General Medicine Prostheses and Implants 020601 biomedical engineering Capillaries Perfusion 11548 Clinic for Vascular Surgery biology.protein Rheology Biomedical engineering |
| Zdroj: | The International journal of artificial organs. 44(2) |
| ISSN: | 1724-6040 |
| Popis: | Vascularization of tissue engineered implants is crucial for their survival and integration in the recipient’s body. Pre-vascularized, fibrin-based implants offer a solution since low concentration fibrin hydrogels (1 mg/mL) have been shown to promote tube formation of endothelial cells in co-culture with adipogenic stem cells. However, higher fibrinogen concentrations (> 20 mg/mL) enabling the fabrication of stable implants are necessary. We here characterized fibrin gels of 1–30 mg/mL for their rheological properties and whether they support tube formation of endothelial cell–adipogenic stem cell co-cultures for up to 7 days. Moreover, 20 mg/mL gels containing preformed channels and endothelial cell–adipogenic stem cell co-culture were perfused continuously in a customized flow chamber with 3.9 dyn/cm2 for 12 days and analyzed for capillary formation. Rheology of fibrin gels showed increasing stability proportional to fibrinogen concentration with 20 mg/mL gels having a storage module of 465 Pa. Complex tube networks stable for 7 days were observed at 1–5 mg/mL gels whereas higher concentrations showed initial sprouting only. However, perfusion of 20 mg/mL fibrin gels resulted in endothelialized pore formation in several layers of the gel with endothelial cell–adipogenic stem cell co-culture. Thus, perfusion supports the formation of capillary-like structures in fibrin gels that are too dense for spontaneous tube formation under static conditions. Future studies are necessary to further increase pore density and to investigate proper nutrition of tissue-specific target cells in the scaffold. |
| Databáze: | OpenAIRE |
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