Reconstitution of a voltage and calcium dependent potassium channel from rat cerebellum
| Autor: | Michela Ottolia, E Babini, P Gazzotti, Lourival D. Possani, Gianfranco Prestipino |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Potassium Channels Charybdotoxin Androctonus australis Potassium Lipid Bilayers Scorpion Venoms chemistry.chemical_element Calcium complex mixtures Membrane Potentials SK channel chemistry.chemical_compound Cerebellum Internal medicine medicine Animals Molecular Biology biology General Neuroscience Tetraethylammonium biology.organism_classification Calcium-activated potassium channel Potassium channel Rats Electrophysiology Endocrinology chemistry Biophysics Neurology (clinical) Ion Channel Gating Developmental Biology |
| Zdroj: | Brain Research. 815:410-413 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/s0006-8993(98)01165-2 |
| Popis: | Membrane vesicles from rat cerebellum were reconstituted into lipid bilayers. The activity of two different potassium channels was recorded: (1) a small conducting voltage dependent potassium channel insensitive to [Ca2+]i, (2) a calcium and voltage dependent potassium channel (KCa). KCa channels had a conductance of (302±15) pS (n=5) and were activated by [Ca2+]i and membrane depolarizations. They were blocked by tetraethylamonium (TEA) and charybdotoxin (CTX) but insensitive to noxiustoxin (NTX). Finally, we showed the blocking effect of Androctonus australis Hector (AaH) scorpion venom on KCa channels from rat cerebellum. |
| Databáze: | OpenAIRE |
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