Engineering temporal accumulation of a low recalcitrance polysaccharide leads to increased C6 sugar content in plant cell walls

Autor:	Miguel E. Vega-Sánchez, Yves Verhertbruggen, Henrik Vibe Scheller, Joshua L. Heazlewood, Pamela C. Ronald, Jesper Harholt, Thomas Herter, Berit Ebert, Jeemeng Lao, Michela Catena, Jay D. Keasling, Fan Yang, Edward E. K. Baidoo, Dominique Loqué
Rok vydání:	2015
Předmět:	chemistry.chemical_classification Aging biology food and beverages Plant Science Genetically modified crops Plants Genetically Modified Polysaccharide biology.organism_classification Mixed-linkage glucan Cell wall chemistry Biochemistry Cell Wall Polysaccharides Plant Cells Botany Arabidopsis thaliana Hordeum vulgare Glucans Agronomy and Crop Science Secondary cell wall Biotechnology Glucan
Zdroj:	Plant Biotechnology Journal. 13:903-914
ISSN:	1467-7652 1467-7644
Popis:	Reduced cell wall recalcitrance and increased C6 monosaccharide content are desirable traits for future biofuel crops, as long as these biomass modifications do not significantly alter normal growth and development. Mixed-linkage glucan (MLG), a cell wall polysaccharide only present in grasses and related species among flowering plants, is comprised of glucose monomers linked by both β-1,3 and β-1,4 bonds. Previous data have shown that constitutive production of MLG in barley (Hordeum vulgare) severely compromises growth and development. Here, we used spatio-temporal strategies to engineer Arabidopsis thaliana plants to accumulate significant amounts of MLG in the cell wall by expressing the rice CslF6 MLG synthase using secondary cell wall and senescence-associated promoters. Results using secondary wall promoters were suboptimal. When the rice MLG synthase was expressed under the control of a senescence-associated promoter, we obtained up to four times more glucose in the matrix cell wall fraction and up to a 42% increase in saccharification compared to control lines. Importantly, these plants grew and developed normally. The induction of MLG deposition at senescence correlated with an increase of gluconic acid in cell wall extracts of transgenic plants in contrast to the other approaches presented in this study. MLG produced in Arabidopsis has an altered structure compared to the grass glucan, which likely affects its solubility, while its molecular size is unaffected. The induction of cell wall polysaccharide biosynthesis in senescing tissues offers a novel engineering alternative to enhance cell wall properties of lignocellulosic biofuel crops.
Databáze:	OpenAIRE
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