Human platelet antigen-1 (Zw) typing using PCR-RFLP
| Autor: | H. O. Madsen, N. Grunnet, J. Georgsen, A. Svejgaard, B. R. Andersen, E. Taaning |
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| Rok vydání: | 1993 |
| Předmět: |
Platelet disorder
Molecular Sequence Data Enzyme-Linked Immunosorbent Assay Prenatal diagnosis Polymerase Chain Reaction Sensitivity and Specificity law.invention Serology law medicine Humans Antigens Human Platelet Typing Polymerase chain reaction Base Sequence biology Histocompatibility Testing Integrin beta3 Reproducibility of Results Hematology medicine.disease Human platelet antigen Immunology Neonatal alloimmune thrombocytopenia biology.protein Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Transfusion Medicine. 3:153-156 |
| ISSN: | 1365-3148 0958-7578 |
| Popis: | Summary. The agreement between human platelet antigen-1 typing with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and typing with a serological ELISA method was evaluated. A total of 82 individuals were typed and an absolute correlation was found between the two typing methods. The PCR-RFLP typing method could be clinically useful in a number of immunologically mediated platelet disorders, characterized by severe thrombocytopenia and in early prenatal diagnosis of neonatal alloimmune thrombocytopenia, in which serological methods are difficult to apply because of their dependency on access to platelets and access to well-characterized anti-HPA-lb antisera. |
| Databáze: | OpenAIRE |
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