Maternal exposure to di-n-butyl phthalate induces alterations of c-Myc gene, some apoptotic and growth related genes in pups' testes
| Autor: | Eman M. Gouda, Mona K. Galal, Adel M El-Behairy, Said Z. Moussa, Maha M. Rashad |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Offspring Health Toxicology and Mutagenesis Genes myc Apoptosis 010501 environmental sciences Biology Toxicology 01 natural sciences Andrology 03 medical and health sciences chemistry.chemical_compound Downregulation and upregulation Pregnancy Testis Animals Rats Wistar Gene 0105 earth and related environmental sciences Public Health Environmental and Occupational Health Phthalate Methylation Fold change Dibutyl Phthalate Rats 030104 developmental biology chemistry Maternal Exposure Female Corn oil |
| Zdroj: | Toxicology and industrial health. 34(11) |
| ISSN: | 1477-0393 |
| Popis: | The aim of this study was to investigate the effects of maternal exposure to di-( n-butyl) phthalate (DBP) on testicular development and function in pre-pubertal and post-pubertal male rat offspring. Fourteen pregnant female rats were equally divided into two groups: a control group and a DBP-treated group. During gestation day (GD) 12 to postnatal day (PND) 14, the control group was administered 1 ml/day corn oil, and the DBP-treated group was administered DBP 500 mg/kg/day by oral gavage. On PND 25 (pre-puberty) and PND 60 (post-puberty), blood for serum and the testes were collected from five male offspring of each group. To determine the relationship between the methylation state of the c-Myc promoter and the expression of the c-Myc gene, some apoptotic-related genes, such as p53 and Bax, the anti-apoptotic Bcl-2 gene, and some growth arrest-related genes, such as BRD7 and GAS1, were examined. Compared with the control ( p < 0.05), at pre-puberty, DBP induces c-Myc hyper-methylation with significant downregulation for c-Myc, p53, Bax genes, and significant upregulation for Bcl-2, BRD7, and GAS1, while at post puberty, the methylation state and expression of c-Myc and apoptosis-related genes returned to control levels in the same sequence with the fold change in the expression of BRD7 and GAS1 genes. These findings suggest that DBP induced a transient pre-pubertal increase in c-Myc promoter methylation that may be associated with disruption of both apoptotic and growth mechanisms in the testes. |
| Databáze: | OpenAIRE |
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