Molecular Epidemiology of an SHV-5 Extended-Spectrum -Lactamase in Enterobacteriaceae Isolated from Infants in a Neonatal Intensive Care Unit
| Autor: | Marilyn A. Kacica, Karen E. Preston, Ronald J. Limberger, Frank J. Scarano, Wendy A. Archinal, Timothy P. Root, Richard A. Venezia, Lynn Steele |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Microbiology (medical)
Neonatal intensive care unit Ceftazidime beta-Lactamases Disease Outbreaks Microbiology law.invention Fatal Outcome Plasmid law Klebsiella polycyclic compounds medicine Humans Cloning Molecular Cross Infection Cephalosporin Resistance Molecular epidemiology biology Infant Newborn Infant Klebsiella oxytoca biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Enterobacteriaceae Intensive care unit Bacterial Typing Techniques Cephalosporins Klebsiella Infections Infectious Diseases Genes Bacterial Intensive Care Neonatal bacteria Isoelectric Focusing Bacteria Plasmids medicine.drug |
| Zdroj: | Clinical Infectious Diseases. 21:915-923 |
| ISSN: | 1537-6591 1058-4838 |
| Popis: | Klebsiella oxytoca that produced extended-spectrum beta-lactamase (ESBL) and were resistant to ceftazidime were isolated from infants in a neonatal intensive care unit (NICU). During a 30-week period, 3 infants developed infections and an additional 60 infants were colonized with these bacteria. The molecular typing data suggested transmission of a single strain of ceftazidime-resistant K. oxytoca among 48 of the 63 infants. The ESBL of 46 of the 48 similar isolates, 14 of the remaining 15 isolates, and 6 other Enterobacteriaceae appeared to be associated with a conjugative plasmid of approximately 85 kb. The ESBL gene was cloned, and DNA sequencing confirmed that the ESBL was an SHV-5. Hybridization data suggested that the SHV-5 gene was transmitted to other Enterobacteriaceae in vivo. The spread of the ESBL was reduced through adherence to infection control practices. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|