Diagnosis of EML4-ALK Translocation With FISH, Immunohistochemistry, and Real-time Polymerase Chain Reaction in Patients With Non-Small Cell Lung Cancer
| Autor: | Vicente Morales-Oyarvide, Carlos Enrique Rojas-Marín, Andrés-Felipe Cardona, Ana María Espinosa-García, Laura Alejandra Ramírez-Tirado, Graciela Cruz-Rico, Manuel Segura-González, Alejandro Avilés-Salas, Oscar Arrieta |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Adult Male Cancer Research Pathology medicine.medical_specialty Lung Neoplasms Oncogene Proteins Fusion In situ hybridization Adenocarcinoma Real-Time Polymerase Chain Reaction law.invention 03 medical and health sciences 0302 clinical medicine law Predictive Value of Tests hemic and lymphatic diseases Carcinoma Non-Small-Cell Lung medicine Anaplastic lymphoma kinase Humans Lung cancer Polymerase chain reaction In Situ Hybridization Fluorescence Aged Neoplasm Staging medicine.diagnostic_test business.industry Middle Aged medicine.disease Immunohistochemistry 030104 developmental biology Real-time polymerase chain reaction Oncology Molecular Diagnostic Techniques 030220 oncology & carcinogenesis Cancer research Female business Fluorescence in situ hybridization |
| Zdroj: | American journal of clinical oncology. 40(6) |
| ISSN: | 1537-453X |
| Popis: | To assess anaplastic lymphoma kinase (ALK) rearrangement detection with immunohistochemistry (IHC) and real-time polymerase chain reaction (RT-qPCR) in comparison with fluorescence in situ hybridization (FISH).Tumor tissue samples from 230 patients with advanced non-small cell lung cancer (NSCLC) were analyzed by FISH to detect ALK rearrangements. Additional IHC tests using 5A4 clone and RT-qPCR (variants 1 to 5) were performed in 63 and 48 patients, respectively.Thirteen percent of FISH tests were not evaluable. From the remaining tests (n=200), 18 (9.0%) were ALK positive (ALK). ALK patients were significantly younger at the time of diagnosis (below 55 y, 14.3% vs. 5.5%, P=0.035), were light smokers (tobacco index10, 12.6% vs. 4.1%, P=0.049), and presented adenocarcinoma with a mucinous component (30.8 vs. 8.0%, P=0.007). When comparing FISH with IHC using a cutoff of 1+ or 2+, and only 2+ staining intensity, the sensitivity, specificity, negative predictive value, and positive predictive value were as follows: 83.3%, 100.0%, 93.75%, and 100.0%; and 55.6%, 100.0%, 84.9%, and 100.0%, respectively. For RT-qPCR, these results were 55.6, 100, 90.7, and 100.0%, respectively.Our results suggest that RT-qPCR is an inadequate initial test for detecting ALK-positive lung cancer. IHC is highly useful as an initial screening test for ALK rearrangement detection in NSCLC. These results contribute to the medical literature on the establishment of IHC as a standard diagnostic test for ALK rearrangements in NSCLC. |
| Databáze: | OpenAIRE |
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