Characterization of the medically important yeast Trichosporon mucoides and its close sister Trichosporon dermatis by traditional and advanced technologies
| Autor: | Sung-Oui Suh, Kendra M. Grosso, Janice L. Houseknecht, Miguel E. Carrion |
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| Rok vydání: | 2015 |
| Předmět: |
Microbiological Techniques
Microbiology (medical) Posaconazole Antifungal Agents Molecular Sequence Data Microbial Sensitivity Tests Biology DNA Ribosomal Microbiology DNA sequencing Mycological Typing Techniques Trichosporon DNA Ribosomal Spacer medicine Cluster Analysis Humans Internal transcribed spacer Trichosporon mucoides DNA Fungal Phylogeny Voriconazole Sequence Analysis DNA General Medicine biology.organism_classification Yeast RNA Ribosomal Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization medicine.drug |
| Zdroj: | Journal of Medical Microbiology. 64:1135-1143 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/jmm.0.000134 |
| Popis: | Trichosporon dermatis is a causative agent of several mycoses in immunocompromised patients but is often misidentified as Trichosporon mucoides due to their phenotypic resemblance. In order to evaluate the current identification keys for these species and to develop a rapid and reliable identification method, 11 strains of these yeasts were fully characterized in this study by traditional and advanced technologies. DNA sequences of the internal transcribed spacer (ITS), IGS1, and D1/D2 regions identified six of the yeasts as T. dermatis that were previously known as T. mucoides, including ATCC 204094 that has been used as the quality-control strain of T. mucoides for the VITEK 2 system and other commercial yeast identification kits. These two species could not be differentiated reliably by any previously known phenotypic keys for the species, such as growth patterns on ethylamine, phloroglucinol and tyramine, or by the VITEK 2 system. On the other hand, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) proved to be a rapid and reliable identification tool for the two closely related yeasts. With newly added superspectra from fully authenticated reference strains, the VITEK MS system using MALDI-TOF MS successfully separated strains of T. dermatis and T. mucoides at a similarity level of approximately 67 % for the mass spectra data, and could identify these strains at the species level with 100 % accuracy in repeated tests. Furthermore, the in vitro susceptibility results indicated that itraconazole, posaconazole and voriconazole were more effective against both T. mucoides and T. dermatis than the other antifungal agents tested in this study. |
| Databáze: | OpenAIRE |
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