Small diameter helical vascular scaffolds support endothelial cell survival
| Autor: | Vijay Parikh, Araida Hidalgo Bastida, Rameen Shakur, Mohammad Sanami, Juned Kadiwala, Cathy M. Holt, May Azzawi, Mohsen Miraftab |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Scaffold food.ingredient Biomedical Engineering Nanofibers Pharmaceutical Science Medicine (miscellaneous) Bioengineering 030204 cardiovascular system & hematology Polyvinyl alcohol Gelatin 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine food medicine Cell Adhesion Humans General Materials Science Cells Cultured Cell Proliferation Tissue Engineering Tissue Scaffolds Cell adhesion molecule Endothelial Cells Coronary Vessels Electrospinning Blood Vessel Prosthesis Endothelial stem cell 030104 developmental biology medicine.anatomical_structure chemistry Gene Expression Regulation Molecular Medicine Mechanosensitive channels Biomedical engineering Artery |
| Zdroj: | Parikh, V, Kadiwala, J, Hidalgo Bastida, A, Holt, C, Sanami, M, Miraftab, M, Shakur, R & Azzawi, M 2018, ' Small diameter helical vascular scaffolds support endothelial cell survival ', Nanomedicine: Nanotechnology, Biology, and Medicine, vol. 14, no. 8, pp. 2598-2608 . https://doi.org/10.1016/j.nano.2018.08.005 |
| ISSN: | 1549-9642 |
| DOI: | 10.1016/j.nano.2018.08.005 |
| Popis: | There is an acute clinical need for small-diameter vascular grafts as a treatment option for cardiovascular disease. Here, we used an intelligent design system to recreate the natural structure and hemodynamics of small arteries. Nano-fibrous tubular scaffolds were fabricated from blends of polyvinyl alcohol and gelatin with inner helices to allow a near physiological spiral flow profile, using the electrospinning technique. Human coronary artery endothelial cells (ECs) were seeded on the inner surface and their viability, distribution, gene expression of mechanosensitive and adhesion molecules compared to that in conventional scaffolds, under static and flow conditions. We show significant improvement in cell distribution in helical vs. conventional scaffolds (94% ± 9% vs. 82% ± 7.2%; P |
| Databáze: | OpenAIRE |
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