Overexpression of 3-ketoacyl-acyl-carrier protein synthase IIIs in plants reduces the rate of lipid synthesis
| Autor: | Jan G. Jaworski, Patricia Edwards, James Byrne, Katayoon Dehesh, Heeyoung Tai |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Spinacia
DNA Complementary Physiology Nicotiana tabacum Molecular Sequence Data Plant Science Gene Expression Regulation Enzymologic chemistry.chemical_compound Biosynthesis Gene Expression Regulation Plant 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase Tobacco Genetics Plant Oils Arabidopsis thaliana Amino Acid Sequence DNA Primers Plant Proteins Base Sequence Sequence Homology Amino Acid Acyl carrier protein synthase biology ATP synthase Reverse Transcriptase Polymerase Chain Reaction food and beverages Plants Plants Genetically Modified biology.organism_classification Lipids Kinetics Fatty acid synthase Biochemistry chemistry biology.protein Spinach lipids (amino acids peptides and proteins) Sequence Alignment Research Article |
| Zdroj: | Plant physiology. 125(2) |
| ISSN: | 1532-2548 0032-0889 |
| Popis: | A cDNA coding for 3-ketoacyl-acyl-carrier protein (ACP) synthase III (KAS III) from spinach (Spinacia oleracea; So KAS III) was used to isolate two closely related KAS III clones (Ch KAS III-1 and Ch KAS III-2) from Cuphea hookeriana. Both Ch KAS IIIs are expressed constitutively in all tissues examined. An increase in the levels of 16:0 was observed in tobacco (Nicotiana tabacum, WT-SR) leaves overexpressing So KAS III when under the control of the cauliflower mosaic virus-35S promoter and in Arabidopsis and rapeseed (Brassica napus)seeds overexpressing either of the Ch KAS IIIs driven by napin. These data indicate that this enzyme has a universal role in fatty acid biosynthesis, irrespective of the plant species from which it is derived or the tissue in which it is expressed. The transgenic rapeseed seeds also contained lower levels of oil as compared with the wild-type levels. In addition, the rate of lipid synthesis in transgenic rapeseed seeds was notably slower than that of the wild-type seeds. The results of the measurements of the levels of the acyl-ACP intermediates as well as any changes in levels of other fatty acid synthase enzymes suggest that malonyl-ACP, the carbon donor utilized by all the 3- ketoacyl-ACP synthases, is limiting in the transgenic plants. This further suggests that malonyl-coenzyme A is a potential limiting factor impacting the final oil content as well as further extension of 16:0. |
| Databáze: | OpenAIRE |
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