Amelioration of Mucositis in Proton Therapy of Fanconi Anemia Fanca−/− Mice by JP4-039
| Autor: | Craig W. Stevens, Peyman Kabolizadeh, Darcy Franicola, Peter Wipf, Stephanie Thermozier, Michael W. Epperly, George S. Wilson, Xuanfeng Ding, Andrew Henderson, Katie Buelow, Joel S. Greenberger, Xiaoqiang Li, Thomas J. Quinn, Aranee Sivananthan |
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| Rok vydání: | 2019 |
| Předmět: |
Mucositis
Senescence congenital hereditary and neonatal diseases and abnormalities Cancer Research Stromal cell Radiation-Protective Agents Radiation Tolerance General Biochemistry Genetics and Molecular Biology Cell Line Mice 03 medical and health sciences 0302 clinical medicine Fanconi anemia hemic and lymphatic diseases Proton Therapy medicine Animals Pharmacology Fanconi Anemia Complementation Group A Protein Chemistry nutritional and metabolic diseases Mesenchymal Stem Cells medicine.disease FANCA Haematopoiesis Fanconi Anemia medicine.anatomical_structure 030220 oncology & carcinogenesis Toxicity Cancer research Nitrogen Oxides Bone marrow Research Article |
| Zdroj: | In Vivo. 33:1757-1766 |
| ISSN: | 1791-7549 0258-851X |
| DOI: | 10.21873/invivo.11666 |
| Popis: | Background/Aim: We tested JP4-039, a GS-nitroxide radiation damage mitigator in proton therapy of Fanconi anemia (FA) mice. Materials and Methods: Fanca(–/–) and Fanca(+/+) bone marrow stromal cells were pre-treated with JP4-039 and irradiated with either protons or photons (0-10 GyRBE) followed by clonogenic survival and β-Galactosidase senescence analysis. Fanca(–/–) and Fanca(+/+) mice were pretreated with JP4-039 for 10 min prior to oropharyngeal irradiation with either protons or photons (0 or 30 GyRBE) followed by sacrifice and measurement of oral cavity ulceration, distant hematopoietic suppression, and real-time polymerase chain reaction analysis. Results: JP4-039 reduced oral cavity ulceration in Fanca(–/–) mice, transcripts Nfkb, Ap1, Sp1, and Nrf2, and proton therapy induced distant marrow suppression. Conclusion: JP4-039 protected Fanca(–/–) and Fanca(+/+) cells and mouse oral cavity from both proton and photon radiation. |
| Databáze: | OpenAIRE |
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