Intralaboratory comparison of analytical methods for quantification of major phytocannabinoids
| Autor: | Petr Tarkowski, Michal Berčák, Tibor Béres, Tomáš Gucký, Lucie Černochová, Sandra Benická, Sanja Ćavar Zeljković |
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| Rok vydání: | 2019 |
| Předmět: |
02 engineering and technology
Mass spectrometry 01 natural sciences Biochemistry Gas Chromatography-Mass Spectrometry Analytical Chemistry law.invention Limit of Detection Tandem Mass Spectrometry law Flame ionization detector Sample preparation Chromatography High Pressure Liquid Cannabis Flame Ionization Chromatography Intralaboratory Cannabinoids Chemistry 010401 analytical chemistry Reproducibility of Results 021001 nanoscience & nanotechnology Mass spectrometric 0104 chemical sciences Gas chromatography Laboratories 0210 nano-technology |
| Zdroj: | Analytical and Bioanalytical Chemistry. 411:3069-3079 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-019-01760-y |
| Popis: | This study compares alternative approaches for analyzing phytocannabinoids in different plant materials. Three chromatographic analytical methods (ultra-high-performance liquid chromatography with tandem mass spectrometric detection and gas chromatography with mass spectrometric and flame ionization detection) were evaluated regarding selectivity, sensitivity, analytical accuracy, and precision. The performance of the methods was compared and all three methods were demonstrated to be appropriate tools for analyzing phytocannabinoids in cannabis. Gas chromatography coupled with mass spectrometric detection showed slightly better accuracy in determining phytocannabinoid acids, which are often difficult to quantify owing to their limited stability. Aspects of sample preparation, such as material homogenization and extraction, were also considered. A single ultrasonic-assisted ethanolic extraction of dried and powdered plant samples of cannabis was shown to be exhaustive for extracting the samples prior to analysis. |
| Databáze: | OpenAIRE |
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