Morphological and Functional Evaluation of Murine Heterotopic Cardiac Grafts Using Ultrasound Biomicroscopy
| Autor: | Lori J. West, Yu-Qing Zhou, Wei Zhu, Kesheng Tao, R. Mark Henkelman, Akiva Feintuch, Rami Bishay, Fraser Golding |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Survival Status medicine.medical_specialty Transplantation Heterotopic Acoustics and Ultrasonics Heart Ventricles Microscopy Acoustic Biophysics Ultrasound biomicroscopy Pulmonary Artery Mice Left coronary artery Coronary Circulation Internal medicine medicine.artery Ascending aorta medicine Animals Mitral Valve Stenosis Radiology Nuclear Medicine and imaging Aorta Mice Inbred C3H Functional evaluation Radiological and Ultrasound Technology business.industry Ultrasound Heart Coronary Vessels Echocardiography Doppler Transplantation Models Animal cardiovascular system Cardiology Heart Transplantation Female Wall thickness business Blood Flow Velocity |
| Zdroj: | Ultrasound in Medicine & Biology. 33:870-879 |
| ISSN: | 0301-5629 |
| DOI: | 10.1016/j.ultrasmedbio.2006.10.016 |
| Popis: | This study investigated the use of an ultrasound biomicroscope (UBM) to observe murine heterotopic cardiac transplants. By using an UBM (30 MHz), cardiac isografts in eight mice were studied on days 1, 5, 14 and 50 posttransplantation. The same method was tested in allografts in two mice on days 1, 5, 7 and 9. Two-dimensional imaging delineated the graft structures with high spatial resolution. In isografts, M-mode recording showed gradually decreased left ventricular (LV) wall thickness and chamber dimension, but increased LV fractional shortening. Doppler sampling measured blood velocities from the ascending aorta, left coronary artery (LCA), aortic and mitral orifices of grafts. In isografts, LCA forward flow caused by native circulation to perfuse the graft myocardium increased from day 1 to 5, then moderately decreased by day 14 and stabilized thereafter. In allografts, LCA forward flow sharply decreased to almost zero between day 5-9. Therefore, UBM is a reliable method for following the survival status of cardiac grafts in mice. |
| Databáze: | OpenAIRE |
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