Homogeneous Visual and Fluorescence Detection of Circulating Tumor Cells in Clinical Samples via Selective Recognition Reaction and Enzyme-Free Amplification
| Autor: | Yunjin Bai, He Yaqin, Yi Xie, Ronghui Zhou, Walter Hu, Jie Chen, Yue Wang, Mei Li, Juan Zhou, Binwu Ying, Runlian Qu, Tangyuheng Liu, Wu Peng, Jia Geng, Jin Huang, Piaopiao Chen, Ke Huang, Xiu Wang |
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| Rok vydání: | 2021 |
| Předmět: |
Lung Neoplasms
Aptamer General Physics and Astronomy 02 engineering and technology 010402 general chemistry 01 natural sciences Circulating tumor cell Limit of Detection Quantum Dots Cadmium Compounds medicine Humans General Materials Science Lung cancer Whole blood A549 cell Chemistry Mucin-1 Mucin General Engineering Neoplastic Cells Circulating 021001 nanoscience & nanotechnology medicine.disease Molecular biology Fluorescence 0104 chemical sciences Spectrometry Fluorescence Naked eye Tellurium 0210 nano-technology |
| Zdroj: | ACS Nano. 15:11634-11643 |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/acsnano.1c02080 |
| Popis: | Here we report a simple all-nucleic-acid enzyme-free catalyzed hairpin assembly assisted amplification strategy with quantum dots (QDs) as the nanoscale signal reporter for homogeneous visual and fluorescent detection of A549 lung cancer cells from clinical blood samples. This work was based on the phenomenon that CdTe QDs can selectively recognize Agsup+/supand C-Agsup+/sup-C and by using mucin 1 as the circulating tumor cells (CTCs) marker and aptamer as the recognition probe. Under optimized conditions, the limits of detections as low as 0.15 fg/mL of mucin 1 and 3 cells/mL of A549 cells were achieved with fluorescence signals. A 1 fg/mL concentration of mucin 1 and 100 cells/mL of A549 can be distinguished by the naked eye. This method was used to quantitatively analyze CTCs in 51 clinical whole blood samples of patients with lung cancer. The levels of CTCs detected in clinical samples by this method were consistent with those obtained using the folate receptor-polymerase chain reaction clinical test kit and correlated with radiologic and pathological findings. |
| Databáze: | OpenAIRE |
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