Enhancement of adaptive response in peanut hairy root by exogenous signalling molecules under cadmium stress
| Autor: | Pimpimon Lumpa, Kanitha Supdensong, Anupan Kongbangkerd, Piyanuch Anuwan, Apinun Limmongkon, Vijakhana Pilaisangsuree |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arachis Physiology Plant Science Cyclopentanes Acetates Real-Time Polymerase Chain Reaction 01 natural sciences Plant Roots 03 medical and health sciences chemistry.chemical_compound Biosynthesis Stress Physiological Tandem Mass Spectrometry Stilbenes Oxylipins Transcription factor Gene chemistry.chemical_classification Methyl jasmonate Phenylpropanoid biology Adaptation Physiological Cell biology 030104 developmental biology Enzyme chemistry Hairy root culture Chitinase biology.protein Transcriptome Agronomy and Crop Science 010606 plant biology & botany Cadmium Chromatography Liquid Signal Transduction |
| Zdroj: | Journal of plant physiology. 254 |
| ISSN: | 1618-1328 |
| Popis: | Plants counteract Cd toxicity by activating cellular stress responses. The simultaneous exogenous application of methyl jasmonate (MeJA) and methyl-β-cyclodextrin (CD) before Cd exposure improved the response of Arachis hypogaea hairy root culture to the unfavourable effects of Cd toxicity. At 24 h after elicitation, genes that encode key enzymes in the phenylpropanoid biosynthesis pathway (i.e., PAL and RS3) were up-regulated to 3.2- and 5.4-fold changes respectively, thereby inducing stilbene production. The up-regulation of genes that encode transcription factors (i.e., ERF1 and ERF6) significantly increased the expression of several genes (PR4A, PR5, PR10, and chitinase) that encode the pathogenesis-related (PR) proteins to 25.8-, 45-, 5- and 12.6-fold changes, respectively. The more dramatic up-regulation of PR protein-encoding genes demonstrated the significant role of defence proteins in plant protective mechanisms. The prolonged (i.e., 72-h) treatment with MeJA + CD_Cd triggered adaptive responses by substantially increasing the levels of antioxidants, stilbenes, and other phenolic substances. These findings suggest that the interaction between signalling elicitors (MeJA and CD) and Cd modulates a complex signalling network for plant defence system. |
| Databáze: | OpenAIRE |
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