Transcriptomic Profiling of the Allorecognition Response to Grafting in the Demosponge Amphimedon queenslandica
| Autor: | Bernard M. Degnan, Laura F. Grice |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Pharmaceutical Science RNA-Seq chemical and pharmacologic phenomena 010603 evolutionary biology 01 natural sciences Article Transcriptome sponge 03 medical and health sciences allorecognition Demosponge Drug Discovery Animals histocompatibility invertebrates Porifera self-nonself recognition Allorecognition Pharmacology Toxicology and Pharmaceutics (miscellaneous) lcsh:QH301-705.5 biology Gene Expression Profiling Anatomy biology.organism_classification Amphimedon queenslandica Cell biology Sponge 030104 developmental biology Body plan surgical procedures operative Gene Expression Regulation lcsh:Biology (General) |
| Zdroj: | Marine Drugs; Volume 15; Issue 5; Pages: 136 Marine Drugs, Vol 15, Iss 5, p 136 (2017) Marine Drugs |
| ISSN: | 1660-3397 |
| DOI: | 10.3390/md15050136 |
| Popis: | Sponges, despite their simple body plan, discriminate between self and nonself with remarkable specificity. Sponge grafting experiments simulate the effects of natural self or nonself contact under laboratory conditions. Here we take a transcriptomic approach to investigate the temporal response to self and nonself grafts in the marine demosponge Amphimedon queenslandica. Auto- and allografts were established, observed and sampled over a period of three days, over which time the grafts either rejected or accepted, depending on the identity of the paired individuals, in a replicable and predictable manner. Fourteen transcriptomes were generated that spanned the auto- and allograft responses. Self grafts fuse completely in under three days, and the process appears to be controlled by relatively few genes. In contrast, nonself grafting results in a complete lack of fusion after three days, and appears to involve a broad downregulation of normal biological processes, rather than the mounting of an intense defensive response. |
| Databáze: | OpenAIRE |
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