Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome
| Autor: | Anne Kathrine W. Runge, Edouard Masson-MacLean, Jessica Hendy, Krista McGrath, Enrico Cappellini, Camilla Speller, Kristine Korzow Richter, Matthew J. Collins, Kate Britton, Meaghan Mackie |
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| Přispěvatelé: | Collins, Matthew [0000-0003-4226-5501], Apollo - University of Cambridge Repository, Runge, Anne Kathrine W. [0000-0003-2421-4831], Richter, Kristine K. [0000-0003-3591-6900], Speller, Camilla [0000-0001-7128-9903] |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
dogs Proteome Range (biology) Niche Zoology Biology General Biochemistry Genetics and Molecular Biology zooms palaeoproteomics 03 medical and health sciences Dogs Research articles Animals 0601 history and archaeology European commission Zooarchaeology General Environmental Science Zooms 060102 archaeology General Immunology and Microbiology Host (biology) Arctic Regions Palaeofaeces Palaeoproteomics archaeology Hominidae 06 humanities and the arts General Medicine The arctic Diet 030104 developmental biology Archaeology Palaeobiology Nunalleq Alaska palaeofaeces General Agricultural and Biological Sciences Alaska |
| Zdroj: | Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Proceedings of the Royal Society B: Biological Sciences Runge, A K W, Hendy, J, Richter, K K, Masson-MacLean, E, Britton, K, MacKie, M, McGrath, K, Collins, M, Cappellini, E & Speller, C 2021, ' Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome ', Proceedings of the Royal Society B: Biological Sciences, vol. 288, no. 1954, 20210020 . https://doi.org/10.1098/rspb.2021.0020 |
| ISSN: | 1471-2954 |
| DOI: | 10.1098/rspb.2021.0020 |
| Popis: | The domestic dog has inhabited the anthropogenic niche for at least 15 000 years, but despite their impact on human strategies, the lives of dogs and their interactions with humans have only recently become a subject of interest to archaeologists. In the Arctic, dogs rely exclusively on humans for food during the winter, and while stable isotope analyses have revealed dietary similarities at some sites, deciphering the details of provisioning strategies have been challenging. In this study, we apply zooarchaeology by mass spectrometry (ZooMS) and liquid chromatography tandem mass spectrometry to dog palaeofaeces to investigate protein preservation in this highly degradable material and obtain information about the diet of domestic dogs at the Nunalleq site, Alaska. We identify a suite of digestive and metabolic proteins from the host species, demonstrating the utility of this material as a novel and viable substrate for the recovery of gastrointestinal proteomes. The recovered proteins revealed that the Nunalleq dogs consumed a range of Pacific salmon species (coho, chum, chinook and sockeye) and that the consumed tissues derived from muscle and bone tissues as well as roe and guts. Overall, the study demonstrated the viability of permafrost-preserved palaeofaeces as a unique source of host and dietary proteomes. 1. Introduction 2. Material and methods (a) Samples (b) Palaeoproteomics (c) Zooarchaeology by mass spectrometry 3. Results and discussion (a) Metaproteomics (b) Host proteins (c) Dietary proteins (d) Challenges and future directions 4. Conclusion |
| Databáze: | OpenAIRE |
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