Population Dynamics between Erwinia amylovora, Pantoea agglomerans and Bacteriophages: Exploiting Synergy and Competition to Improve Phage Cocktail Efficacy
| Autor: | Alan J. Castle, Michael Parcey, Antonet M. Svircev, Steven Gayder, Darlene Nesbitt |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
bacteriophages phage therapy Phage therapy medicine.medical_treatment viruses Population Myoviridae Erwinia medicine.disease_cause Microbiology 03 medical and health sciences Podoviridae phage cocktails Virology medicine phage carrier education lcsh:QH301-705.5 030304 developmental biology fire blight 0303 health sciences education.field_of_study biology 030306 microbiology phage–host dynamics Pathogenic bacteria biology.organism_classification Pantoea agglomerans 3. Good health qPCR Lytic cycle lcsh:Biology (General) |
| Zdroj: | Microorganisms Volume 8 Issue 9 Microorganisms, Vol 8, Iss 1449, p 1449 (2020) |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms8091449 |
| Popis: | Bacteriophages are viruses capable of recognizing with high specificity, propagating inside of, and destroying their bacterial hosts. The phage lytic life cycle makes phages attractive as tools to selectively kill pathogenic bacteria with minimal impact on the surrounding microbiome. To effectively harness the potential of phages in therapy, it is critical to understand the phage&ndash host dynamics and how these interactions can change in complex populations. Our model examined the interactions between the plant pathogen Erwinia amylovora, the antagonistic epiphyte Pantoea agglomerans, and the bacteriophages that infect and kill both species. P. agglomerans strains are used as a phage carrier their role is to deliver and propagate the bacteriophages on the plant surface prior to the arrival of the pathogen. Using liquid cultures, the populations of the pathogen, carrier, and phages were tracked over time with quantitative real-time PCR. The jumbo Myoviridae phage ϕEa35-70 synergized with both the Myoviridae ϕEa21-4 and Podoviridae ϕEa46-1-A1 and was most effective in combination at reducing E. amylovora growth over 24 h. Phage ϕEa35-70, however, also reduced the growth of P. agglomerans. Phage cocktails of ϕEa21-4, ϕEa46-1-A1, and ϕEa35-70 at multiplicities of infections (MOIs) of 10, 1, and 0.01, respectively, no longer inhibited growth of P. agglomerans. When this cocktail was grown with P. agglomerans for 8 h prior to pathogen introduction, pathogen growth was reduced by over four log units over 24 h. These findings present a novel approach to study complex phage&ndash host dynamics that can be exploited to create more effective phage-based therapies. |
| Databáze: | OpenAIRE |
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