Control of calcium oxalate crystal structure and cell adherence by urinary macromolecules
| Autor: | Elaine M. Worcester, John H. Wiessner, Jack G. Kleinman, Neil S. Mandel, Jeffrey A. Wesson |
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| Rok vydání: | 1998 |
| Předmět: |
crystal structure
stone formation 030232 urology & nephrology Calcium oxalate chemistry.chemical_element Crystal structure Calcium Oxalate law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine law Cell Adhesion medicine Humans Crystallization 030304 developmental biology 0303 health sciences Kidney Calcium Oxalate medicine.disease epithelial cells urine Kidney Tubules medicine.anatomical_structure chemistry Biochemistry Polymorphism (materials science) Nephrology Urinary Calculi Kidney stones Protein Binding nephrolithiasis |
| Zdroj: | Kidney International. 53:952-957 |
| ISSN: | 0085-2538 |
| DOI: | 10.1111/j.1523-1755.1998.00839.x |
| Popis: | Control of calcium oxalate crystal structure and cell adherence by urinary macromolecules. Crystal polymorphism is exhibited by calcium oxalates in nephrolithiasis, and we have proposed that a shift in the preferred crystalline form of calcium oxalate (CaOx) from monohydrate (COM) to dihydrate (COD) induced by urinary macromolecules reduces crystal attachment to epithelial cell surfaces, thus potentially inhibiting a critical step in the genesis of kidney stones. We have tested the validity of this hypothesis by studying both the binding of monohydrate and dihydrate crystals to renal tubule cells and the effect of macromolecular urinary solutes on crystal structure. Renal tubule cells grown in culture bound 50% more CaOx monohydrate than dihydrate crystals of comparable size. The effects of macromolecules on the spontaneous nucleation of CaOx were examined in HEPES-buffered saline solutions containing Ca2+ and C2O42- at physiologic concentrations and supersaturation. Many naturally occurring macromolecules known to be inhibitors of crystallization, specifically osteopontin, nephrocalcin and urinary prothrombin fragment 1, were found to favor the formation of calcium oxalate dihydrate in this in vitro system, while other polymers did not affect CaOx crystal structure. Thus, the natural defense against nephrolithiasis may include impeding crystal attachment by an effect of macromolecular inhibitors on the preferred CaOx crystal structure that forms in urine. |
| Databáze: | OpenAIRE |
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