CK1 Is Required for a Mitotic Checkpoint that Delays Cytokinesis
| Autor: | Kathleen L. Gould, Alyssa E. Johnson, Jun-Song Chen |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Cell cycle checkpoint
M Phase Cell Cycle Checkpoints Green Fluorescent Proteins Cell Cycle Proteins Protein Serine-Threonine Kinases Biology Article General Biochemistry Genetics and Molecular Biology Spindle pole body 03 medical and health sciences 0302 clinical medicine Schizosaccharomyces CHFR Phosphorylation Protein kinase A Mitosis Cytokinesis 030304 developmental biology 0303 health sciences Agricultural and Biological Sciences(all) Biochemistry Genetics and Molecular Biology(all) Casein Kinase I Ubiquitination Polo kinase Nuclear Proteins Cell biology Spindle Pole Bodies 030220 oncology & carcinogenesis Schizosaccharomyces pombe Proteins General Agricultural and Biological Sciences Microtubule-Associated Proteins Protein Kinases Cell Division Signal Transduction |
| Zdroj: | Current Biology. (19):1920-1926 |
| ISSN: | 0960-9822 |
| DOI: | 10.1016/j.cub.2013.07.077 |
| Popis: | Summary Failure to accurately partition genetic material during cell division causes aneuploidy and drives tumorigenesis [1]. Cell-cycle checkpoints safeguard cells from such catastrophes by impeding cell-cycle progression when mistakes arise. FHA-RING E3 ligases, including human RNF8 [2] and CHFR [3] and fission yeast Dma1 [4], relay checkpoint signals by binding phosphorylated proteins via their FHA domains and promoting ubiquitination of downstream targets [5]. Upon mitotic checkpoint activation, S. pombe Dma1 concentrates at spindle pole bodies (SPBs) in an FHA-dependent manner and ubiquitinates Sid4, a scaffold of Polo kinase, to suspend cytokinesis [6]. However, the kinase or kinases that phosphoprime Sid4 for Dma1-mediated ubiquitination are unknown. Here, we report that the highly conserved protein kinase CK1 transmits the signal necessary to stall cytokinesis by phosphopriming Sid4 for Dma1-mediated ubiquitination. Like Dma1, CK1 accumulates at SPBs during a mitotic arrest and associates stably with SPB components, including Sid4. Our results establish CK1 as an integral component of a mitotic, ubiquitin-mediated checkpoint pathway. |
| Databáze: | OpenAIRE |
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