Oxidized Polyethylene Wax as a Potential Carbon Source for PHA Production
| Autor: | Guozhan Jiang, Marek Kowalczuk, Michał Kwiecień, Iza Radecka, Brian Johnston, Adam A. Marek, David Hill, Craig D. Williams, Grazyna Adamus, Jan Zawadiak, Victor U. Irorere |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Thermogravimetric analysis medium chain length PHA (mcl-PHA) Materials science Electrospray ionization polyhydroxyalkanoates oxidized PE wax Ralstonia eutropha H16 mass spectrometry 02 engineering and technology Mass spectrometry Tandem mass spectrometry lcsh:Technology Article Polyhydroxyalkanoates Gel permeation chromatography 03 medical and health sciences chemistry.chemical_compound Differential scanning calorimetry Organic chemistry General Materials Science lcsh:Microscopy lcsh:QC120-168.85 Chromatography lcsh:QH201-278.5 lcsh:T 021001 nanoscience & nanotechnology equipment and supplies 6. Clean water 030104 developmental biology chemistry lcsh:TA1-2040 Tryptone lcsh:Descriptive and experimental mechanics lcsh:Electrical engineering. Electronics. Nuclear engineering lcsh:Engineering (General). Civil engineering (General) 0210 nano-technology lcsh:TK1-9971 |
| Zdroj: | Materials; Volume 9; Issue 5; Pages: 367 Materials, Vol 9, Iss 5, p 367 (2016) Materials |
| ISSN: | 1996-1944 |
| DOI: | 10.3390/ma9050367 |
| Popis: | We report on the ability of bacteria to produce biodegradable polyhydroxyalkanoates (PHA) using oxidized polyethylene wax (O-PEW) as a novel carbon source. The O-PEW was obtained in a process that used air or oxygen as an oxidizing agent. R. eutropha H16 was grown for 48 h in either tryptone soya broth (TSB) or basal salts medium (BSM) supplemented with O-PEW and monitored by viable counting. Study revealed that biomass and PHA production was higher in TSB supplemented with O-PEW compared with TSB only. The biopolymers obtained were preliminary characterized by nuclear magnetic resonance (NMR), gel permeation chromatography (GPC), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The detailed structural evaluation at the molecular level was performed by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The study revealed that, when TSB was supplemented with O-PEW, bacteria produced PHA which contained 3-hydroxybutyrate and up to 3 mol % of 3-hydroxyvalerate and 3-hydroxyhexanoate co-monomeric units. The ESI-MS/MS enabled the PHA characterization when the content of 3-hydroxybutyrate was high and the appearance of other PHA repeating units was very low. |
| Databáze: | OpenAIRE |
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