A large inversion in the linear chromosome of Streptomyces griseus caused by replicative transposition of a new Tn3 family transposon
| Autor: | M. Murata, Alexander Lezhava, Tetsuya Uchida, Haruyasu Kinashi, Yingjie Yang |
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| Rok vydání: | 2011 |
| Předmět: |
DNA
Bacterial Transposable element Subfamily Retroelements Replicative transposition Molecular Sequence Data Restriction Mapping Biochemistry Microbiology Genome Transposition (music) Genetics Molecular Biology Chromosomal inversion Base Sequence biology Streptomyces griseus Chromosome Sequence Analysis DNA General Medicine Chromosomes Bacterial biology.organism_classification Chromosome Inversion Mutation bacteria Transposon Resolvases |
| Zdroj: | Archives of Microbiology. 193:299-306 |
| ISSN: | 1432-072X 0302-8933 |
| DOI: | 10.1007/s00203-010-0674-5 |
| Popis: | We have comprehensively analyzed the linear chromosomes of Streptomyces griseus mutants constructed and kept in our laboratory. During this study, macrorestriction analysis of AseI and DraI fragments of mutant 402-2 suggested a large chromosomal inversion. The junctions of chromosomal inversion were cloned and sequenced and compared with the corresponding target sequences in the parent strain 2247. Consequently, a transposon-involved mechanism was revealed. Namely, a transposon originally located at the left target site was replicatively transposed to the right target site in an inverted direction, which generated a second copy and at the same time caused a 2.5-Mb chromosomal inversion. The involved transposon named TnSGR was grouped into a new subfamily of the resolvase-encoding Tn3 family transposons based on its gene organization. At the end, terminal diversity of S. griseus chromosomes is discussed by comparing the sequences of strains 2247 and IFO13350. |
| Databáze: | OpenAIRE |
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