Anticancer potency of nitric oxide-releasing liposomes
| Autor: | Mark H. Schoenfisch, Dakota J. Suchyta |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Liposome medicine.diagnostic_test Chemistry General Chemical Engineering General Chemistry Molecular biology Article Flow cytometry Nitric oxide 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Apoptosis Cancer cell Drug delivery medicine Cytotoxicity Intracellular |
| Zdroj: | RSC Advances. 7:53236-53246 |
| ISSN: | 2046-2069 |
| Popis: | In this study, fast and slow nitric oxide (NO)-releasing liposomes (half-lives of 2.5 and >72 h, respectively) were prepared by encapsulation of N-propyl-1,3-propanediamine/NO (PAPA/NO) and diethylenetriamine/NO (DETA/NO), respectively, via reverse phase evaporation. The anticancer activity of the otherwise equivalent fast and slow NO-releasing systems was evaluated against several distinct pancreatic, colorectal, and breast cancer cell lines. The anticancer assays (via cytotoxicity) over 72 h revealed that the slower NO-releasing liposomes consistently required lower NO payloads (LD50 6 μg/mL) to elicit cytotoxicity. The mechanism of intracellular NO build-up in cancer cells was studied using confocal fluorescence microscopy and flow cytometry, the results of which indicated that a more gradual NO accumulation was characteristic of the slow NO-release system. Protein expression via Western blot analysis revealed that slower NO release resulted in more necrotic/apoptotic cells, while faster release reduced the number of mitotic cells to a greater extent. Overall, these studies demonstrate the potential of NO-releasing liposomes for anticancer therapy and highlight the significance of release kinetics (and NO payloads) required to induce cell death. |
| Databáze: | OpenAIRE |
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