Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide
| Autor: | Paula S. Salgado, James A. Garnett, Jonathan D. Taylor, Ernesto Cota, Bing Liu, Yingqi Xu, Sebastian Lambert, Steve Matthews, Jing Lin, Wei-chao Lee |
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| Rok vydání: | 2012 |
| Předmět: |
inorganic chemicals
Salmonella enteritidis Fimbria Biophysics Crystallography X-Ray Biochemistry Article Fimbriae Proteins law.invention Microbiology law Deuterium Oxide Crystallization Molecular Biology biology Chemistry Cell Biology Protein Structure Tertiary Bacterial adhesin Deuterium Fimbriae Bacterial Pilin biology.protein Protein Multimerization Cell Adhesion Molecules Macromolecule |
| Zdroj: | Biochemical and Biophysical Research Communications. 421:208-213 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2012.03.136 |
| Popis: | The use of heavy water (D(2)O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D(2)O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD. |
| Databáze: | OpenAIRE |
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